A1CLD2 · BUD32_ASPCL
- ProteinEKC/KEOPS complex subunit bud32
- Genebud32
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids277 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.
Catalytic activity
- L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 26-34 | ATP (UniProtKB | ChEBI) | |||
Binding site | 49 | ATP (UniProtKB | ChEBI) | |||
Active site | 172 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome, telomeric region | |
Cellular Component | cytosol | |
Cellular Component | EKC/KEOPS complex | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | histone H2AS1 kinase activity | |
Molecular Function | hydrolase activity | |
Molecular Function | protein serine kinase activity | |
Biological Process | protein phosphorylation | |
Biological Process | tRNA processing | |
Biological Process | tRNA threonylcarbamoyladenosine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEKC/KEOPS complex subunit bud32
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Fumigati
Accessions
- Primary accessionA1CLD2
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000278905 | 1-277 | EKC/KEOPS complex subunit bud32 | ||
Keywords
- PTM
Interaction
Subunit
Component of the EKC/KEOPS complex composed of at least Bud32, cgi121, gon7, kae1 and pcc1; the whole complex dimerizes.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-20 | Pro residues | |||
Region | 1-23 | Disordered | |||
Domain | 20-277 | Protein kinase | |||
Domain
This protein is considered an atypical serine/threonine kinase, because it lacks the conventional structural elements necessary for the substrate recognition as well as a lysine residue that in all other serine/threonine kinases participates in the catalytic event (By similarity).
BUD32 has protein kinase activity in vitro, but in the context of the EKC/KEOPS complex, the catalytic subunit KAE1 switches the activity of BUD32 from kinase into ATPase (By similarity).
BUD32 has protein kinase activity in vitro, but in the context of the EKC/KEOPS complex, the catalytic subunit KAE1 switches the activity of BUD32 from kinase into ATPase (By similarity).
Sequence similarities
Belongs to the protein kinase superfamily. BUD32 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)31,061
- Last updated2007-01-23 v1
- ChecksumC334D54533C62B09
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-20 | Pro residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DS027056 EMBL· GenBank· DDBJ | EAW09956.1 EMBL· GenBank· DDBJ | Genomic DNA |