A1A1Q5 · TSAD_BIFAA
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- GenetsaD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids347 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Catalytic activity
- adenosine37 in tRNA + L-threonylcarbamoyladenylate = AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 113 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 117 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 136-140 | substrate | ||||
Sequence: IVSGG | ||||||
Binding site | 170 | substrate | ||||
Sequence: D | ||||||
Binding site | 183 | substrate | ||||
Sequence: G | ||||||
Binding site | 187 | substrate | ||||
Sequence: D | ||||||
Binding site | 282 | substrate | ||||
Sequence: N | ||||||
Binding site | 310 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Bifidobacteriales > Bifidobacteriaceae > Bifidobacterium
Accessions
- Primary accessionA1A1Q5
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000303280 | 1-347 | tRNA N6-adenosine threonylcarbamoyltransferase | |||
Sequence: MSEPIVLGIESTCDETAAAVVQGRTLISNVVASSMDEHARYGGVIPEIASRAHAEAFVPCVSKALADANMTLSDVDAIAVSAGPGLAGCLAVGVSGAKSLAWAANKPIYGINHVIGHIAVTQLQFGPFPKDTLALIVSGGHTSLLHVEDVARHIDVVGTTLDDAAGECFDKVARLLGFPYPGGPHIDRHAQLGNPHAIKVPQGLTQGKAGQQHPYDFSFSGVKTAVARWIEQQQAEGNEIPIDDVCASLADSVATVLARKAMRGCEQYGSKTLIVGGGFSANSQLRAKLLEVGAKHGVEVRIPQIKLCTDNGAMVAMLGVNLVESGVKPSEPDFAIDSAMPLTKISM |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length347
- Mass (Da)36,127
- Last updated2007-01-23 v1
- ChecksumD55D37B4823A57FD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP009256 EMBL· GenBank· DDBJ | BAF39638.1 EMBL· GenBank· DDBJ | Genomic DNA |