A0ZSF4 · MAP1_MIZYE
- ProteinNacrein-like protein P1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids331 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Acts as a negative regulator for calcification in the shells of mollusks. May function both as a calcium concentrator and as a carbonic anhydrase required for production of carbonate ions, which are assembled to CaCO3 at mineralization sites. Is important for shell formation in both the calcitic prismatic layer and the aragonitic nacreous layer (By similarity).
Shows inhibitory activity of crystal formation when present in free state but, when attached to the insoluble matrix, may regulate the form and size of aragonite crystal
Shows inhibitory activity of crystal formation when present in free state but, when attached to the insoluble matrix, may regulate the form and size of aragonite crystal
Catalytic activity
- H+ + hydrogencarbonate = CO2 + H2O
Cofactor
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | carbonate dehydratase activity | |
Molecular Function | zinc ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNacrein-like protein P1
- EC number
Organism names
- Taxonomic lineageEukaryota > Metazoa > Spiralia > Lophotrochozoa > Mollusca > Bivalvia > Autobranchia > Pteriomorphia > Pectinida > Pectinoidea > Pectinidae > Mizuhopecten
Accessions
- Primary accessionA0ZSF4
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000379793 | 1-331 | Nacrein-like protein P1 | |||
Sequence: QSPINIVSYDAKFRQRLPKLKFKPHMEKLKTEVTNHQNRAPEFEPEDGENLYVKLNNLVDGHYKFHNLHVHNGRTRRKGSEHSVNGRFTPMEAHLVFHHDDQTHFEPTRTKLGGAFPGHNDFVVVGVFLEVGDDGFGDEPDDEECKRILKGHHPDNNENGNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGENGNNGENGNNGENGNNGENGHKHGCRVKKAKHLSTILECAYRNDKVREFKKVGEEEGLDVHLTPEMPLPPLKNRHYYTYEGSLTTPPCTESVLWVVQKCHVQVSRRVLHALRNVEGY |
Keywords
- PTM
Expression
Tissue specificity
Expressed in the mantle.
Interaction
Subunit
Homooligomer; disulfide-linked. May also be disulfide-linked to insoluble organic matrix (By similarity).
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-331 | Alpha-carbonic anhydrase | ||||
Sequence: QSPINIVSYDAKFRQRLPKLKFKPHMEKLKTEVTNHQNRAPEFEPEDGENLYVKLNNLVDGHYKFHNLHVHNGRTRRKGSEHSVNGRFTPMEAHLVFHHDDQTHFEPTRTKLGGAFPGHNDFVVVGVFLEVGDDGFGDEPDDEECKRILKGHHPDNNENGNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGENGNNGENGNNGENGNNGENGHKHGCRVKKAKHLSTILECAYRNDKVREFKKVGEEEGLDVHLTPEMPLPPLKNRHYYTYEGSLTTPPCTESVLWVVQKCHVQVSRRVLHALRNVEGY | ||||||
Region | 138-240 | Disordered | ||||
Sequence: DEPDDEECKRILKGHHPDNNENGNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGENGNNGENGNNGENGNNGENGHKHGCR | ||||||
Compositional bias | 141-157 | Basic and acidic residues | ||||
Sequence: DDEECKRILKGHHPDNN | ||||||
Compositional bias | 158-231 | Polar residues | ||||
Sequence: ENGNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGENGNNGENGNNGENGNNG | ||||||
Repeat | 162-164 | 1 | ||||
Sequence: GDN | ||||||
Region | 162-233 | 24 X 3 AA approximate tandem repeats of G-X-N | ||||
Sequence: GDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGENGNNGENGNNGENGNNGEN | ||||||
Repeat | 165-167 | 2 | ||||
Sequence: GNN | ||||||
Repeat | 168-170 | 3 | ||||
Sequence: GYN | ||||||
Repeat | 171-173 | 4 | ||||
Sequence: GDN | ||||||
Repeat | 174-176 | 5 | ||||
Sequence: GNN | ||||||
Repeat | 177-179 | 6 | ||||
Sequence: GDN | ||||||
Repeat | 180-182 | 7 | ||||
Sequence: GNN | ||||||
Repeat | 183-185 | 8 | ||||
Sequence: GYN | ||||||
Repeat | 186-188 | 9 | ||||
Sequence: GDN | ||||||
Repeat | 189-191 | 10 | ||||
Sequence: GNN | ||||||
Repeat | 192-194 | 11 | ||||
Sequence: GDN | ||||||
Repeat | 195-197 | 12 | ||||
Sequence: GNN | ||||||
Repeat | 198-200 | 13 | ||||
Sequence: GYN | ||||||
Repeat | 201-203 | 14 | ||||
Sequence: GDN | ||||||
Repeat | 204-206 | 15 | ||||
Sequence: GNN | ||||||
Repeat | 207-209 | 16 | ||||
Sequence: GDN | ||||||
Repeat | 210-212 | 17 | ||||
Sequence: GNN | ||||||
Repeat | 213-215 | 18 | ||||
Sequence: GEN | ||||||
Repeat | 216-218 | 19 | ||||
Sequence: GNN | ||||||
Repeat | 219-221 | 20 | ||||
Sequence: GEN | ||||||
Repeat | 222-224 | 21 | ||||
Sequence: GNN | ||||||
Repeat | 225-227 | 22 | ||||
Sequence: GEN | ||||||
Repeat | 228-229 | 23 | ||||
Sequence: GN | ||||||
Repeat | 231-233 | 24 | ||||
Sequence: GEN |
Domain
The Gly-Xaa-Asn repeat domain binds calcium.
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Length331
- Mass (Da)36,918
- Last updated2007-01-23 v1
- Checksum48E96EDDB73DCDAB
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: Q | ||||||
Compositional bias | 141-157 | Basic and acidic residues | ||||
Sequence: DDEECKRILKGHHPDNN | ||||||
Compositional bias | 158-231 | Polar residues | ||||
Sequence: ENGNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGYNGDNGNNGDNGNNGENGNNGENGNNGENGNNG | ||||||
Non-terminal residue | 331 | |||||
Sequence: Y |