A0SVK0 · DOG1_ARATH
- ProteinProtein DELAY OF GERMINATION 1
- GeneDOG1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids291 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for the induction of seed dormancy (PubMed:17065317, PubMed:22829147, PubMed:26620523).
The level of DOG1 protein in freshly harvested seeds determines the level of seed dormancy (PubMed:22829147).
Determines the temperature window for germination by regulating the expression of micropylar endosperm-weakening genes through temperature control of the gibberellins metabolism (PubMed:25114251).
Regulates seed dormancy and flowering time through an influence on levels of microRNAs miR156 and miR172 (PubMed:27035986).
Regulator of seed maturation interfering with abscisic acid signaling components and activating ABI5 (PubMed:26729600).
In cv. Cvi-1, enhances glucose induction of ABI4 (PubMed:18410483).
The level of DOG1 protein in freshly harvested seeds determines the level of seed dormancy (PubMed:22829147).
Determines the temperature window for germination by regulating the expression of micropylar endosperm-weakening genes through temperature control of the gibberellins metabolism (PubMed:25114251).
Regulates seed dormancy and flowering time through an influence on levels of microRNAs miR156 and miR172 (PubMed:27035986).
Regulator of seed maturation interfering with abscisic acid signaling components and activating ABI5 (PubMed:26729600).
In cv. Cvi-1, enhances glucose induction of ABI4 (PubMed:18410483).
Miscellaneous
The Columbia and Landsberg erecta alleles have weak dormancy phenotypes, whereas the Cvi-1 allele shows a strong dormancy phenotype (PubMed:17065317).
The Cvi-1 allele shows a sugar-supersensitive phenotype, whereas the Columbia and Landsberg erecta alleles don't have a significant effect on sugar sensitivity (PubMed:18410483).
The Cvi-1 allele shows a sugar-supersensitive phenotype, whereas the Columbia and Landsberg erecta alleles don't have a significant effect on sugar sensitivity (PubMed:18410483).
Transgenic mutant seeds expressing single DOG1 transcript variants lack dormancy and do not accumulate DOG1 protein. Simultaneous expression of two or more DOG1 transcript variants encoding different isoforms, however, leads to the accumulation of DOG1 protein and increased seed dormancy (PubMed:26684465).
The spliceosome disassembly factor STIP1/NTR1 is required for proper transcript levels and splicing of DOG1 (PubMed:25568310).
The spliceosome disassembly factor STIP1/NTR1 is required for proper transcript levels and splicing of DOG1 (PubMed:25568310).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | identical protein binding | |
Molecular Function | sequence-specific DNA binding | |
Biological Process | abscisic acid-activated signaling pathway | |
Biological Process | DNA-templated transcription | |
Biological Process | regulation of seed dormancy process | |
Biological Process | seed dormancy process | |
Biological Process | sugar mediated signaling pathway |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein DELAY OF GERMINATION 1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionA0SVK0
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
Loss of seed dormancy, but no effect on sugar sensitivity (in cv. Columbia) (PubMed:17065317, PubMed:18410483).
A T-DNA insertion mutant lacking the long version of the DOG1 transcript (isoform 1) but expressing isoform 2 exhibits stronger seed dormancy (PubMed:26620523).
A T-DNA insertion mutant lacking the long version of the DOG1 transcript (isoform 1) but expressing isoform 2 exhibits stronger seed dormancy (PubMed:26620523).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 15 | Strongly reduced self-binding. | ||||
Sequence: Y → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 62 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000437685 | 1-291 | Protein DELAY OF GERMINATION 1 | |||
Sequence: MGSSSKNIEQAQDSYLEWMSLQSQRIPELKQLLAQRRSHGDEDNDNKLRKLTGKIIGDFKNYAAKRADLAHRCSSNYYAPTWNSPLENALIWMGGCRPSSFFRLVYALCGSQTEIRVTQFLRNIDGYESSGGGGGASLSDLSAEQLAKINVLHVKIIDEEEKMTKKVSSLQEDAADIPIATVAYEMENVGEPNVVVDQALDKQEEAMARLLVEADNLRVDTLAKILGILSPVQGADFLLAGKKLHLSMHEWGTMRDRRRRDCMVDTEVIFDACTTVNSGPRPTETTNNERN |
Post-translational modification
A shift in isoelectric focusing of the protein occurs during after-ripening, probably leading to its loss of function.
Proteomic databases
Expression
Tissue specificity
Detected only in seeds (PubMed:17065317).
Expressed mainly in vascular tissues of the cotyledon, hypocotyl and radicle of the embryo
Expressed mainly in vascular tissues of the cotyledon, hypocotyl and radicle of the embryo
Induction
Up-regulation by glucose of the Cvi-1 allele, but not of the Columbia and Landsberg erecta alleles (PubMed:18410483).
May be up-regulated by the transcript elongation factor TFIIS (PubMed:21569772).
Up-regulated by cold (PubMed:21803937, PubMed:22829147).
Up-regulated by histone monoubiquitination through HUB1 (PubMed:17329563).
Down-regulated by the histone demethylases LDL1 and LDL2 (PubMed:25852712).
May be up-regulated by the transcript elongation factor TFIIS (PubMed:21569772).
Up-regulated by cold (PubMed:21803937, PubMed:22829147).
Up-regulated by histone monoubiquitination through HUB1 (PubMed:17329563).
Down-regulated by the histone demethylases LDL1 and LDL2 (PubMed:25852712).
Developmental stage
Isoform 1 and isoform 2: Transcription starts at the beginning of seed maturation 9 days after pollination and reaches its highest level during the last phases of seed development (PubMed:17065317, PubMed:22829147, PubMed:26620523).
Isoform 1: Upon imbibition the transcripts rapidly disappear in both dormant and after-ripened seeds, but the level of protein is hardly affected (PubMed:17065317, PubMed:22829147).
Isoform 2: Transcription starts at the beginning of seed maturation 9 days after pollination and reaches its highest level during the last phases of seed development (PubMed:26620523).
Isoform 1: Upon imbibition the transcripts rapidly disappear in both dormant and after-ripened seeds, but the level of protein is hardly affected (PubMed:17065317, PubMed:22829147).
Isoform 2: Transcription starts at the beginning of seed maturation 9 days after pollination and reaches its highest level during the last phases of seed development (PubMed:26620523).
Gene expression databases
Interaction
Subunit
Homodimer (PubMed:26684465).
Self-binding does not influence protein accumulation but is required for full function (PubMed:26684465).
Single isoforms are functional, but the presence of additional isoforms is required to prevent protein degradation (PubMed:26684465).
Self-binding does not influence protein accumulation but is required for full function (PubMed:26684465).
Single isoforms are functional, but the presence of additional isoforms is required to prevent protein degradation (PubMed:26684465).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | A0SVK0 | AHG1 Q9FLI3 | 3 | EBI-25512274, EBI-2363348 | |
BINARY | A0SVK0 | AIP1 Q9LNW3 | 5 | EBI-25512274, EBI-1573499 | |
BINARY | A0SVK0 | At2g29380 Q9ZW21 | 5 | EBI-25512274, EBI-4441103 | |
BINARY | A0SVK0 | DOG1 A0SVK0 | 3 | EBI-25512274, EBI-25512274 | |
BINARY | A0SVK0 | DREB2A O82132 | 3 | EBI-25512274, EBI-1786840 | |
BINARY | A0SVK0 | PP2CA P49598 | 3 | EBI-25512274, EBI-1764934 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-258 | DOG1 | ||||
Sequence: IEQAQDSYLEWMSLQSQRIPELKQLLAQRRSHGDEDNDNKLRKLTGKIIGDFKNYAAKRADLAHRCSSNYYAPTWNSPLENALIWMGGCRPSSFFRLVYALCGSQTEIRVTQFLRNIDGYESSGGGGGASLSDLSAEQLAKINVLHVKIIDEEEKMTKKVSSLQEDAADIPIATVAYEMENVGEPNVVVDQALDKQEEAMARLLVEADNLRVDTLAKILGILSPVQGADFLLAGKKLHLSMHEWGTMRDRR |
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing. A number of isoforms are produced.
A0SVK0-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length291
- Mass (Da)32,470
- Last updated2007-01-09 v1
- Checksum58BC5E3F940889ED
A0SVK0-2
- Name2
- NoteThe use of a proximal polyadenylation site leads to the production of a short DOG1 mRNA that is translated in vivo, producing an isoform that is sufficient for seed dormancy establishment.Produced by alternative polyadenylation.
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8BB61 | A0A1P8BB61_ARATH | DOG1 | 308 | ||
A0A2H1ZE87 | A0A2H1ZE87_ARATH | DOG1 | 323 |
Sequence caution
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EF028469 EMBL· GenBank· DDBJ | ABK81210.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF028472 EMBL· GenBank· DDBJ | ABK81213.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB016870 EMBL· GenBank· DDBJ | BAB09311.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002688 EMBL· GenBank· DDBJ | ANM68819.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | ANM68820.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | ANM68821.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK228646 EMBL· GenBank· DDBJ | BAF00554.1 EMBL· GenBank· DDBJ | mRNA |