A0SVK0 · DOG1_ARATH

Function

function

Required for the induction of seed dormancy (PubMed:17065317, PubMed:22829147, PubMed:26620523).
The level of DOG1 protein in freshly harvested seeds determines the level of seed dormancy (PubMed:22829147).
Determines the temperature window for germination by regulating the expression of micropylar endosperm-weakening genes through temperature control of the gibberellins metabolism (PubMed:25114251).
Regulates seed dormancy and flowering time through an influence on levels of microRNAs miR156 and miR172 (PubMed:27035986).
Regulator of seed maturation interfering with abscisic acid signaling components and activating ABI5 (PubMed:26729600).
In cv. Cvi-1, enhances glucose induction of ABI4 (PubMed:18410483).

Miscellaneous

The Columbia and Landsberg erecta alleles have weak dormancy phenotypes, whereas the Cvi-1 allele shows a strong dormancy phenotype (PubMed:17065317).
The Cvi-1 allele shows a sugar-supersensitive phenotype, whereas the Columbia and Landsberg erecta alleles don't have a significant effect on sugar sensitivity (PubMed:18410483).
Transgenic mutant seeds expressing single DOG1 transcript variants lack dormancy and do not accumulate DOG1 protein. Simultaneous expression of two or more DOG1 transcript variants encoding different isoforms, however, leads to the accumulation of DOG1 protein and increased seed dormancy (PubMed:26684465).
The spliceosome disassembly factor STIP1/NTR1 is required for proper transcript levels and splicing of DOG1 (PubMed:25568310).

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular Functionidentical protein binding
Molecular Functionsequence-specific DNA binding
Biological Processabscisic acid-activated signaling pathway
Biological ProcessDNA-templated transcription
Biological Processregulation of seed dormancy process
Biological Processseed dormancy process
Biological Processsugar mediated signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein DELAY OF GERMINATION 1
  • Alternative names
    • GLUCOSE SENSING QTL 5

Gene names

    • Name
      DOG1
    • Synonyms
      GSQ5
    • ORF names
      K15I22.3
    • Ordered locus names
      At5g45830

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Cvi-1
    • cv. Kon
    • cv. Landsberg erecta
    • cv. Sha
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    A0SVK0
  • Secondary accessions
    • Q0WQP5
    • Q9FJ53

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Isoform 1

Nucleus

Isoform 2

Nucleus

Keywords

Phenotypes & Variants

Disruption phenotype

Loss of seed dormancy, but no effect on sugar sensitivity (in cv. Columbia) (PubMed:17065317, PubMed:18410483).
A T-DNA insertion mutant lacking the long version of the DOG1 transcript (isoform 1) but expressing isoform 2 exhibits stronger seed dormancy (PubMed:26620523).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis15Strongly reduced self-binding.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 62 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004376851-291Protein DELAY OF GERMINATION 1

Post-translational modification

A shift in isoelectric focusing of the protein occurs during after-ripening, probably leading to its loss of function.

Proteomic databases

Expression

Tissue specificity

Detected only in seeds (PubMed:17065317).
Expressed mainly in vascular tissues of the cotyledon, hypocotyl and radicle of the embryo

Induction

Up-regulation by glucose of the Cvi-1 allele, but not of the Columbia and Landsberg erecta alleles (PubMed:18410483).
May be up-regulated by the transcript elongation factor TFIIS (PubMed:21569772).
Up-regulated by cold (PubMed:21803937, PubMed:22829147).
Up-regulated by histone monoubiquitination through HUB1 (PubMed:17329563).
Down-regulated by the histone demethylases LDL1 and LDL2 (PubMed:25852712).

Developmental stage

Isoform 1 and isoform 2: Transcription starts at the beginning of seed maturation 9 days after pollination and reaches its highest level during the last phases of seed development (PubMed:17065317, PubMed:22829147, PubMed:26620523).
Isoform 1: Upon imbibition the transcripts rapidly disappear in both dormant and after-ripened seeds, but the level of protein is hardly affected (PubMed:17065317, PubMed:22829147).
Isoform 2: Transcription starts at the beginning of seed maturation 9 days after pollination and reaches its highest level during the last phases of seed development (PubMed:26620523).

Gene expression databases

Interaction

Subunit

Homodimer (PubMed:26684465).
Self-binding does not influence protein accumulation but is required for full function (PubMed:26684465).
Single isoforms are functional, but the presence of additional isoforms is required to prevent protein degradation (PubMed:26684465).

Binary interactions

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain8-258DOG1

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing. A number of isoforms are produced.

A0SVK0-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    291
  • Mass (Da)
    32,470
  • Last updated
    2007-01-09 v1
  • Checksum
    58BC5E3F940889ED
MGSSSKNIEQAQDSYLEWMSLQSQRIPELKQLLAQRRSHGDEDNDNKLRKLTGKIIGDFKNYAAKRADLAHRCSSNYYAPTWNSPLENALIWMGGCRPSSFFRLVYALCGSQTEIRVTQFLRNIDGYESSGGGGGASLSDLSAEQLAKINVLHVKIIDEEEKMTKKVSSLQEDAADIPIATVAYEMENVGEPNVVVDQALDKQEEAMARLLVEADNLRVDTLAKILGILSPVQGADFLLAGKKLHLSMHEWGTMRDRRRRDCMVDTEVIFDACTTVNSGPRPTETTNNERN

A0SVK0-2

  • Name
    2
  • Note
    The use of a proximal polyadenylation site leads to the production of a short DOG1 mRNA that is translated in vivo, producing an isoform that is sufficient for seed dormancy establishment.
    Produced by alternative polyadenylation.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8BB61A0A1P8BB61_ARATHDOG1308
A0A2H1ZE87A0A2H1ZE87_ARATHDOG1323

Sequence caution

The sequence BAB09311.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_058562268-276in isoform 2
Alternative sequenceVSP_058563277-291in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EF028469
EMBL· GenBank· DDBJ
ABK81210.1
EMBL· GenBank· DDBJ
Genomic DNA
EF028472
EMBL· GenBank· DDBJ
ABK81213.1
EMBL· GenBank· DDBJ
Genomic DNA
AB016870
EMBL· GenBank· DDBJ
BAB09311.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002688
EMBL· GenBank· DDBJ
ANM68819.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
ANM68820.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
ANM68821.1
EMBL· GenBank· DDBJ
Genomic DNA
AK228646
EMBL· GenBank· DDBJ
BAF00554.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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