A0R4M9 · OTSA_MYCS2

Function

function

Involved in the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P), and probably in the osmoprotection via the biosynthesis of trehalose (PubMed:27513637, Ref.4). Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to glucose-6-phosphate (Glc-6-P) to form trehalose-6-phosphate (Ref.4). ADP-Glc, CDP-Glc, GDP-Glc and TDP-Glc are also glucosyl donors, however, when the pyrimidine sugar nucleotides (CDP-Glc, TDP-Glc and UDP-Glc) are used as substrates, there is an absolute requirement for a high molecular weight polyanion for activity (Ref.4).

Catalytic activity

Activity regulation

Stimulated by the polynucleotide FII (physiological activator), and by chondroitin sulfate (CS) and heparin. Activation by the polyanion is inhibited by high salt concentration as well as by high concentrations of mononucleoside phosphates.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
40 μMUDP-Glcin the presence of polyanion
1 mMGlc-6-Pwith UDP-Glc in the presence of polyanion
2 mMGlc-6-Pwith GDP-Glc in the presence of polyanion

pH Dependence

Optimum pH is 7.

Temperature Dependence

About 60% of the activity is lost in the absence of FII after 10 minutes at 40 degrees Celsius, but only 35% in the presence of FII.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site22D-glucose 6-phosphate (UniProtKB | ChEBI)
Binding site42-43UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site94D-glucose 6-phosphate (UniProtKB | ChEBI)
Site103Involved in alpha anomer selectivity
Binding site148D-glucose 6-phosphate (UniProtKB | ChEBI)
Site173Involved in alpha anomer selectivity
Binding site290UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site295UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site328D-glucose 6-phosphate (UniProtKB | ChEBI)
Binding site393-397UDP-alpha-D-glucose (UniProtKB | ChEBI)

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Trehalose-6-phosphate synthase
  • EC number
  • Short names
    TPS
  • Alternative names
    • Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
    • Osmoregulatory trehalose synthesis protein A
      (OtsA
      )

Gene names

    • Name
      otsA
    • Ordered locus names
      MSMEG_5892, MSMEI_5731

Organism names

Accessions

  • Primary accession
    A0R4M9
  • Secondary accessions
    • I7FLH5

Proteomes

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene do not accumulate ADP-glucose, however combined inactivation of both glgM and ostA accumulates ADP-glucose.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003489101-503Trehalose-6-phosphate synthase

Proteomic databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region481-503Disordered
Compositional bias485-503Polar residues

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    503
  • Mass (Da)
    56,280
  • Last updated
    2007-01-09 v1
  • Checksum
    3D4B5CE2E6DA5ACF
MSPESGHETISGTSDFVVVANRLPVDLERLPDGTTRWKRSPGGLVTALEPLLRKRRGSWIGWAGVADSDEEPIVQDGLQLHPVRLSADDVAKYYEGFSNATLWPLYHDLIVKPEYHREWWDRYVEVNRRFAEATARAAAEGATVWIQDYQLQLVPKMLRMLRPDVTIGFFLHIPFPPVELFMQMPWRTEIVEGLLGADLVGFHLPGGAQNFLVLSRRLVGANTSRASIGVRSRFGEVQVGFRTVKVGAFPISIDSAELDGKARNRAIRQRARQIRAELGNPRKIMLGVDRLDYTKGIDVRLRALSELLEEKRIKRDDTVLVQLATPSRERVESYIAMREDIERQVGHINGEYGEVGHPIVHYLHRPIPRDELIAFFVAADVMLVTPLRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRQAYLVNPHDLEGVKDKIEAAVNQNPEEGKRRMRALRRQVLAHDVDRWARSFLDALAATGETGDSGVTGESTPAPESDSGSF

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias485-503Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000480
EMBL· GenBank· DDBJ
ABK71484.1
EMBL· GenBank· DDBJ
Genomic DNA
CP001663
EMBL· GenBank· DDBJ
AFP42165.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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