A0R3F9 · PAT_MYCS2

Function

function

Catalyzes specifically the acetylation of the epsilon-amino group of a highly conserved lysine residue in acetyl-CoA synthetase (ACS) and of the universal stress protein (USP) MSMEG_4207. Acetylation results in the inactivation of ACS activity and could be important for mycobacteria to adjust to environmental changes.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically regulated by cAMP.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3.1 μMacetyl-CoA (with ACS as substrate)
10 μMacetyl-CoA (with USP as substrate)
10.2 μMACS
335 μMUSP

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site85-883',5'-cyclic AMP (UniProtKB | ChEBI); allosteric activator
Binding site95-963',5'-cyclic AMP (UniProtKB | ChEBI); allosteric activator
Binding site1353',5'-cyclic AMP (UniProtKB | ChEBI); allosteric activator
Binding site211Mg2+ (UniProtKB | ChEBI)
Binding site237-239substrate
Binding site245-250substrate
Binding site276substrate
Binding site285substrate

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular FunctionN-acetyltransferase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acetyltransferase Pat
  • EC number
  • Alternative names
    • GCN5-related N-acetyltransferase (GNAT)
    • Protein acetyltransferase (Pat)

Gene names

    • Ordered locus names
      MSMEG_5458, MSMEI_5308

Organism names

Accessions

  • Primary accession
    A0R3F9

Proteomes

Phenotypes & Variants

Disruption phenotype

Disruption of this gene abolishes acetylation of USP.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis95No increase in the rate of acetylation in the presence of cAMP, presumably because of its inability to bind cAMP.
Mutagenesis234Shows a lower rate of acetylation of USP in the absence of cAMP than the wild-type protein, but in the presence of cAMP, an increase in the rate of acetyltransferase activity is observed.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004203611-333Acetyltransferase Pat

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain153-317N-acetyltransferase

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    333
  • Mass (Da)
    36,848
  • Last updated
    2007-01-09 v1
  • Checksum
    C6CCBC36F639C945
MAELTEVRAADLAALEFFTGCRPSALEPLATQLRPLKAEPGQVLIRQGDPALTFMLIESGRVQVSHAVADGPPIVLDIEPGLIIGEIALLRDAPRTATVVAAEPVIGWVGDRDAFDTILHLPGMFDRLVRIARQRLAAFITPIPVQVRTGEWFYLRPVLPGDVERTLNGPVEFSSETLYRRFQSVRKPTRALLEYLFEVDYADHFVWVMTEGALGPVIADARFVREGHNATMAEVAFTVGDDYQGRGIGSFLMGALIVSANYVGVQRFNARVLTDNMAMRKIMDRLGAVWVREDLGVVMTEVDVPPVDTVPFEPELIDQIRDATRKVIRAVSQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000480
EMBL· GenBank· DDBJ
ABK70370.1
EMBL· GenBank· DDBJ
Genomic DNA
CP001663
EMBL· GenBank· DDBJ
AFP41750.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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