A0R2D3 · RSEA_MYCS2
- ProteinAnti-sigma-E factor RseA
- GenerseA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids132 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
An anti-sigma factor for extracytoplasmic function (ECF) sigma factor SigE. ECF sigma factors are held in an inactive form by an anti-sigma factor (By similarity).
Cofactor
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | metal ion binding |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameAnti-sigma-E factor RseA
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycolicibacterium
Accessions
- Primary accessionA0R2D3
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Disruption phenotype
No phenotype upon growth in liquid culture.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000422951 | 1-132 | Anti-sigma-E factor RseA | |||
Sequence: MADPGHVFRRAFSWLPSQFASQSDAPVGAPRQFGSTEHLSVEAIAAFVDGELRMSAHLRAAHHLSLCPECAAEVDAQSQARTALRESCPIAIPNSLLGMLSQIPHRTPEVTPDVSEQAKFADDPTRGRRKRR | ||||||
Modified residue | 36 | Phosphothreonine; by PknB | ||||
Sequence: T |
Post-translational modification
Phosphorylated by PknB on Thr-36; can be dephosphorylated (at least in vitro) by PstP. Phosphorylation is the signal for subsequent degradation by the ClpC1-ClpP2 complex (By similarity).
Degraded following vancomycin treatment (surface stress) by a ClpC1-ClpP2 complex.
Keywords
- PTM
Proteomic databases
Expression
Induction
Total levels of RseA decrease after detergent or vancomycin treatment of whole cells (at protein level).
Interaction
Subunit
Interacts with ECF RNA polymerase sigma factor SigE, interaction is abrogated by treatment of cells with H2O2, detergent or vancomycin (the latter 2 cause surface stress). This probably inhibits the interaction of SigE with the RNA polymerase catalytic core.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 106-132 | Disordered | ||||
Sequence: RTPEVTPDVSEQAKFADDPTRGRRKRR |
Sequence similarities
Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length132
- Mass (Da)14,426
- Last updated2007-01-09 v1
- Checksum8F94510E3C8C5A1B
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000480 EMBL· GenBank· DDBJ | ABK72380.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001663 EMBL· GenBank· DDBJ | AFP41388.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |