A0R0W9 · TREH_MYCS2

Function

function

Catalyzes the hydrolysis of alpha,alpha-trehalose into two molecules of D-glucose. Does not hydrolyze maltose, isomaltose, sucrose, cellobiose, p-nitrophenyl-alpha-D-glucopyranoside, and methyl-alpha-D-glucopyranoside. Is also inactive on alpha,beta-trehalose, beta,beta-trehalose, alpha,alpha-trehalose-6,6'-dibehenate, trehalulose, nigerose, and trehalose dimycolate.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Shows an absolute requirement for Mg2+ for activity. Mg2+ cannot be replaced by Ca2+, Mn2+ or Zn2+.
phosphate (UniProtKB | Rhea| CHEBI:43474 )

Note: Shows an absolute requirement for inorganic phosphate for activity. The function of the phosphate may involve stabilizing the protein conformation and/or initiating protein aggregation.

Activity regulation

Inhibited by pyrophosphate and polyphosphates. Also competitively inhibited by validoxylamine and castanospermine, but not by trehazolin.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
20 mMalpha,alpha-trehalose

pH Dependence

Optimum pH is 7.1. The trehalase activity drops sharply at pH values of 6.5 and below, as well as at pH values of 8.0 and above.

Pathway

Glycan degradation; trehalose degradation; D-glucose from alpha,alpha-trehalose: step 1/1.

GO annotations

AspectTerm
Molecular Functionalpha,alpha-trehalase activity
Molecular Functionphosphate ion binding
Biological Processtrehalose catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Trehalase
  • EC number
  • Alternative names
    • Alpha,alpha-trehalase
    • Alpha,alpha-trehalose glucohydrolase

Gene names

    • ORF names
      MSMEG4528
    • Ordered locus names
      MSMEG_4535, MSMEI_4422

Organism names

Accessions

  • Primary accession
    A0R0W9
  • Secondary accessions
    • I7GCG8

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004139791-668Trehalase

Proteomic databases

Interaction

Subunit

Homomultimer of 20 or more subunits.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-23Disordered

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    668
  • Mass (Da)
    74,690
  • Last updated
    2007-01-09 v1
  • Checksum
    22AE1581E85F503A
MVLQQTEPTDGADRKASDGPLTVTAPVPYAAGPTLRNPFPPIADYGFLSDCETTCLISSAGSVEWLCVPRPDSPSVFGAILDRGAGHFRLGPYGVSVPAARRYLPGSLILETTWQTHTGWLIVRDALVMGPWHDIDTRSRTHRRTPMDWDAEHILLRTVRCVSGTVELVMSCEPAFDYHRVSATWEYSGPAYGEAIARASRNPDSHPTLRLTTNLRIGIEGREARARTRLTEGDNVFVALSWSKHPAPQTYEEAADKMWKTSEAWRQWINVGDFPDHPWRAYLQRSALTLKGLTYSPTGALLAAPTTSLPETPQGERNWDYRYSWIRDSTFALWGLYTLGLDREADDFFSFIADVSGANNGERHPLQVMYGVGGERSLVEEELHHLSGYDNSRPVRIGNGAYNQRQHDIWGTMLDSVYLHAKSREQIPDALWPVLKNQVEEAIKHWKEPDRGIWEVRGEPQHFTSSKIMCWVALDRGSKLAELQGEKSYAQQWRAIAEEIKADVLARGVDKRGVLTQRYGDDALDASLLLAVLTRFLPADDPRIRATVLAIADELTEDGLVLRYRVEETDDGLAGEEGTFTICSFWLVSALVEIGEISRAKHLCERLLSFASPLHLYAEEIEPRTGRHLGNFPQAFTHLALINAVVHVIRAEEEADSSGVFVPANAPM

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000480
EMBL· GenBank· DDBJ
ABK72415.1
EMBL· GenBank· DDBJ
Genomic DNA
CP001663
EMBL· GenBank· DDBJ
AFP40876.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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