A0QVT2 · RNJ_MYCS2

Function

function

An RNase that has endonuclease and 5'-3' exonuclease activity. The 5'-exonuclease activity acts on 5'-monophosphate but not 5'-triphosphate ends. Endonuclease activity can cleave within 4 nucleotides of the 5'-end of a triphosphorylated RNA. Plays the major role in pre-23S rRNA maturation, and a minor role in processing of pre-5S and pre-16S rRNA.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds up to 2 Zn2+ ions per subunit. It is not clear if Zn2+ or Mg2+ is physiologically important.

Features

Showing features for binding site.

155850100150200250300350400450500550
TypeIDPosition(s)Description
Binding site81Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site83Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site85Zn2+ 2 (UniProtKB | ChEBI); catalytic
Binding site86Zn2+ 2 (UniProtKB | ChEBI); catalytic
Binding site148Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site170Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site170Zn2+ 2 (UniProtKB | ChEBI); catalytic
Binding site371-375substrate
Binding site397Zn2+ 2 (UniProtKB | ChEBI); catalytic

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Molecular Function5'-3' RNA exonuclease activity
Molecular FunctionRNA binding
Molecular FunctionRNA endonuclease activity
Molecular Functionzinc ion binding
Biological ProcessrRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease J
  • EC number
  • Short names
    RNase J

Gene names

    • Name
      rnj
    • Ordered locus names
      MSMEG_2685, MSMEI_2620

Organism names

Accessions

  • Primary accession
    A0QVT2

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Non-essential, it can be deleted. No effect on processing of furA-katG operon mRNA or of pre-5S rRNA processing. Complete loss of mature 23S rRNA. Minor effects on pre-16S rRNA processing. A double rnj-rne depletion mutant indicates this enzyme plays a role in degradation of previously processed pre-16S rRNAs and a decrease in mature 5S rRNA.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis85-86Severely decreases 5'-3' exonuclease activity, retains considerable endonuclease activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004295781-558Ribonuclease J

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    558
  • Mass (Da)
    59,617
  • Last updated
    2007-01-09 v1
  • Checksum
    DE2BF91DEFD4AFF8
MSAELAPPPPLAPGGLRVTALGGISEIGRNMTVFEHLGRLLIVDCGVLFPGHDEPGVDLILPDLRHIEDRLDEIEALVVTHAHEDHIGAIPFLLKLRPDIPVVGSKFTIALVREKCREHRLKPKFVEVAERQSSQHGVFECEYFAVNHSIPGCLAVAIHTGAGTVLHTGDIKLDQLPLDGRPTDLPGMSRLGDAGVDLFLCDSTNSEHPGVSPSESEVGPTLHRLIRGAEGRVIVACFASNVDRVQQIIDAAVALGRRVSFVGRSMVRNMGIARELGYLKVDDSDILDIAAAEMMPPDRVVLITTGTQGEPMAALSRMSRGEHRSITLTSGDLIILSSSLIPGNEEAVYGVIDSLSKIGARVVTNAQARVHVSGHAYAGELLFLYNGVRPRNVMPVHGTWRHLRANAALAASTGVPPENIVLAENGVSVDLVAGRASISGAVTVGKMFVDGLITGDVGDATLGERLILSSGFVSITVVVHRGTGRPAGPAHLISRGFSEDPKALEPVAQKVERELEALAADNVTDPTRIAQAVRRTVGKWVGETYRRQPMIVPTVIEI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000480
EMBL· GenBank· DDBJ
ABK74200.1
EMBL· GenBank· DDBJ
Genomic DNA
CP001663
EMBL· GenBank· DDBJ
AFP39088.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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