A0QQF4 · TTFA_MYCS2
- ProteinTrehalose monomycolate transport factor A
- GenettfA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids278 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for MmpL3-dependent trehalose monomycolate (TMM) transport to the cell wall. Required for growth and cell elongation.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell septum | |
Cellular Component | cell tip | |
Cellular Component | plasma membrane | |
Biological Process | cell wall organization | |
Biological Process | lipid transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameTrehalose monomycolate transport factor A
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycolicibacterium
Accessions
- Primary accessionA0QQF4
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass membrane protein
Note: Colocalizes with MmpL3 to the cell poles and septa. Trehalose monomycolate (TMM) synthesis is not required for localization to the poles or septa.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1 | Periplasmic | ||||
Sequence: M | ||||||
Transmembrane | 2-22 | Helical | ||||
Sequence: VPLWFTLSALCFVGAAVLLYV | ||||||
Topological domain | 23-278 | Cytoplasmic | ||||
Sequence: DIDRRRGLGRRRKSWAKSHGFDYEYESEDLLKRWKRGVMSTVGDVTAKNVVLGQIRGEAVFIFDIEEVATVIALHRKVGTNVVVDLRLKGLKEPRENDIWLLGAIGPRMVYSTNLDAARRACDRRMVTFAHTAPDCAEIMWNEQNWTLVAMPVTSNRAQWDEGLRTVRQFNDLLRVLPPVPQNGSQAALPRRGGSPSRPLAPTPAGRRELPPGRADVPPARGDVSRFAPRPEAGRSDAFRRPPPARNGREASHFQR |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Knockdown of this gene leads to cessation of growth and shorter misshapen cells. Accummulation of trehalose monomycolate (TMM) and lack of trehalose dimycolate (TDM). Accumulates MSMEG_5308 protein.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1-23 | No localization to poles or septa, loss of function complementation and interaction with MmpL3. | ||||
Sequence: Missing | ||||||
Mutagenesis | 24-278 | No localization to poles or septa, loss of function complementation and interaction with MmpL3. | ||||
Sequence: Missing | ||||||
Mutagenesis | 51-278 | No localization to poles or septa, loss of function complementation and interaction with MmpL3. | ||||
Sequence: Missing | ||||||
Mutagenesis | 101-278 | No localization to poles or septa, loss of function complementation and interaction with MmpL3. | ||||
Sequence: Missing | ||||||
Mutagenesis | 151-278 | No localization to poles or septa, loss of function complementation and interaction with MmpL3. | ||||
Sequence: Missing | ||||||
Mutagenesis | 206-278 | Localizes to poles and septa in a pattern similar to the wild-type. Complements function. Interacts with MmpL3. | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452603 | 1-278 | Trehalose monomycolate transport factor A | |||
Sequence: MVPLWFTLSALCFVGAAVLLYVDIDRRRGLGRRRKSWAKSHGFDYEYESEDLLKRWKRGVMSTVGDVTAKNVVLGQIRGEAVFIFDIEEVATVIALHRKVGTNVVVDLRLKGLKEPRENDIWLLGAIGPRMVYSTNLDAARRACDRRMVTFAHTAPDCAEIMWNEQNWTLVAMPVTSNRAQWDEGLRTVRQFNDLLRVLPPVPQNGSQAALPRRGGSPSRPLAPTPAGRRELPPGRADVPPARGDVSRFAPRPEAGRSDAFRRPPPARNGREASHFQR |
Proteomic databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 200-278 | Disordered | ||||
Sequence: PPVPQNGSQAALPRRGGSPSRPLAPTPAGRRELPPGRADVPPARGDVSRFAPRPEAGRSDAFRRPPPARNGREASHFQR | ||||||
Compositional bias | 250-278 | Basic and acidic residues | ||||
Sequence: APRPEAGRSDAFRRPPPARNGREASHFQR |
Domain
The N-terminal domain (1-205) is necessary and sufficient for proper localization and function of this protein, and for its interaction with MmpL3.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length278
- Mass (Da)31,196
- Last updated2007-01-09 v1
- ChecksumB5CD4F2D3C2A7517
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 250-278 | Basic and acidic residues | ||||
Sequence: APRPEAGRSDAFRRPPPARNGREASHFQR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000480 EMBL· GenBank· DDBJ | ABK72891.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001663 EMBL· GenBank· DDBJ | AFP37200.1 EMBL· GenBank· DDBJ | Genomic DNA |