A0QNJ0 · ECCB1_MYCS2
- ProteinESX-1 secretion system ATPase EccB1
- GeneeccB1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids479 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
An ATPase (shown for residues 72-479) (PubMed:26396239).
Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB (PubMed:15687187).
Plays a role in DNA conjugation, in both donor and recipient strains (PubMed:15314236).
Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB (PubMed:15687187).
Plays a role in DNA conjugation, in both donor and recipient strains (PubMed:15314236).
Miscellaneous
DNA conjugation in M.smegmatis is unidirectional with distinct donor and recipient strains; mc2155 is a donor strain while MKD8 is a recipient strain. Mutations in a donor strain that alter DNA transfer do not always alter DNA transfer in a recipient strain.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | hydrolase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameESX-1 secretion system ATPase EccB1
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycolicibacterium
Accessions
- Primary accessionA0QNJ0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-44 | Cytoplasmic | ||||
Sequence: MAGFRLTTKVQVSGWRFLLRRVEHAIVRRDTRMFDDPLQFYSRA | ||||||
Transmembrane | 45-65 | Helical | ||||
Sequence: VFAGVVVSVLICLGAALMAYF | ||||||
Topological domain | 66-479 | Periplasmic | ||||
Sequence: KPLGKQGSDQLLVDRTTNQLYVMLPGSNQLRPVYNLTSARLVLGNASNPVAVKSEELNRISKGQSIGIPGAPYATPTGTPASQWTLCDTVAKPDSSAPKVETSILIRTLAIDSGVGPIRADQGMLVSYEGANWLITEGGRHSIDLADRAVTSAVGIPVTAKPTPISQGLFNALPNRGPWQLPQIPAAGAPNSVGLPENLVIGSVFRTATESDPQHYVVLPDGVARVNNTTAAALRATNSYGLMQPPAVEASVVAKIPEQVYVSPLPDQPLDVLLRQDSPVLCWSWQREPGDQAPKTTVIAGRRLPLPANAIGTGIDQIGGDSTVYIEGGQFVRLQSPDPRVGESMYYIDPQGVRYGIANDDAAKNLGLAGPVNAPWQVVGLLVDGPVLSKEAALIEHDTLPADPNPRKVASGEG |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000438312 | 1-479 | ESX-1 secretion system ATPase EccB1 | |||
Sequence: MAGFRLTTKVQVSGWRFLLRRVEHAIVRRDTRMFDDPLQFYSRAVFAGVVVSVLICLGAALMAYFKPLGKQGSDQLLVDRTTNQLYVMLPGSNQLRPVYNLTSARLVLGNASNPVAVKSEELNRISKGQSIGIPGAPYATPTGTPASQWTLCDTVAKPDSSAPKVETSILIRTLAIDSGVGPIRADQGMLVSYEGANWLITEGGRHSIDLADRAVTSAVGIPVTAKPTPISQGLFNALPNRGPWQLPQIPAAGAPNSVGLPENLVIGSVFRTATESDPQHYVVLPDGVARVNNTTAAALRATNSYGLMQPPAVEASVVAKIPEQVYVSPLPDQPLDVLLRQDSPVLCWSWQREPGDQAPKTTVIAGRRLPLPANAIGTGIDQIGGDSTVYIEGGQFVRLQSPDPRVGESMYYIDPQGVRYGIANDDAAKNLGLAGPVNAPWQVVGLLVDGPVLSKEAALIEHDTLPADPNPRKVASGEG | ||||||
Disulfide bond | 152↔347 | |||||
Sequence: CDTVAKPDSSAPKVETSILIRTLAIDSGVGPIRADQGMLVSYEGANWLITEGGRHSIDLADRAVTSAVGIPVTAKPTPISQGLFNALPNRGPWQLPQIPAAGAPNSVGLPENLVIGSVFRTATESDPQHYVVLPDGVARVNNTTAAALRATNSYGLMQPPAVEASVVAKIPEQVYVSPLPDQPLDVLLRQDSPVLC |
Keywords
- PTM
Proteomic databases
Interaction
Subunit
Part of the ESX-1 / type VII secretion system (T7SS), which is composed of cytosolic and membrane components. The ESX-1 membrane complex is composed of EccB1, EccCa1, EccCb1, EccD1 and EccE1 (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 75-127 | Structural repeat A1/1 | ||||
Sequence: QLLVDRTTNQLYVMLPGSNQLRPVYNLTSARLVLGNASNPVAVKSEELNRISK | ||||||
Region | 187-243 | Structural repeat C1/2 | ||||
Sequence: QGMLVSYEGANWLITEGGRHSIDLADRAVTSAVGIPVTAKPTPISQGLFNALPNRGP | ||||||
Region | 267-323 | Structural repeat A2/3 | ||||
Sequence: GSVFRTATESDPQHYVVLPDGVARVNNTTAAALRATNSYGLMQPPAVEASVVAKIPE | ||||||
Region | 392-447 | Structural repeat C2/4 | ||||
Sequence: EGGQFVRLQSPDPRVGESMYYIDPQGVRYGIANDDAAKNLGLAGPVNAPWQVVGLL |
Domain
Has a discontinuous central core of beta sheets stabilized by a disulfide bond that is flanked by 2 structural domain repeats on each side; the protein forms an elongated 'S'-shaped structure (PubMed:26922638).
Sequence similarities
Belongs to the EccB family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length479
- Mass (Da)51,022
- Last updated2007-01-09 v1
- ChecksumBB7F3268BF10A69B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000480 EMBL· GenBank· DDBJ | ABK72695.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001663 EMBL· GenBank· DDBJ | AFP36543.1 EMBL· GenBank· DDBJ | Genomic DNA |