A0QKC8 · FBIB_MYCA1

Function

function

Bifunctional enzyme that catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives, and the FMNH2-dependent reduction of dehydro-F420-0 to form F420-0.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Monovalent cation. The ion could be potassium.

Pathway

Cofactor biosynthesis; coenzyme F420 biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site21-24GTP (UniProtKB | ChEBI)
Binding site51GTP (UniProtKB | ChEBI)
Binding site56GTP (UniProtKB | ChEBI)
Binding site110a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site113GTP (UniProtKB | ChEBI)
Binding site151a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site152a divalent metal cation 2 (UniProtKB | ChEBI)
Binding site261-265FMN (UniProtKB | ChEBI)
Binding site289FMN (UniProtKB | ChEBI)
Binding site321coenzyme F420-(gamma-Glu)n (UniProtKB | ChEBI)
Binding site400FMN (UniProtKB | ChEBI)
Binding site437FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functioncoenzyme F420-0:L-glutamate ligase activity
Molecular Functioncoenzyme F420-1:gamma-L-glutamate ligase activity
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular Functionoxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
Biological ProcessF420-0 metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional F420 biosynthesis protein FbiB

Including 2 domains:

  • Recommended name
    Coenzyme F420:L-glutamate ligase
  • EC number
  • Alternative names
    • Coenzyme F420-0:L-glutamate ligase
    • Coenzyme F420-1:gamma-L-glutamate ligase
  • Recommended name
    Dehydro-coenzyme F420-0 reductase
  • EC number

Gene names

    • Name
      fbiB
    • Ordered locus names
      MAV_4225

Organism names

  • Taxonomic identifier
  • Strain
    • 104
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)

Accessions

  • Primary accession
    A0QKC8

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000672601-449Bifunctional F420 biosynthesis protein FbiB

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-245Coenzyme F420:L-glutamate ligase
Region246-449Dehydro-coenzyme F420-0 reductase

Sequence similarities

In the N-terminal section; belongs to the CofE family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    449
  • Mass (Da)
    47,396
  • Last updated
    2007-01-09 v1
  • Checksum
    2338D44B7B2B5957
MSPAGEHGTAAPIEILPVAGLPEFRPGDDLGAAVAKAAPWLRDGDVVVVTSKAVSKCEGRLVPAPADPEERDRLRRKLVDEEAVRVLARKGRTLITENRHGLVQAAAGVDGSNVGRDELALLPLDPDASAAALRARLRELLGVEVAVLVTDTMGRAWRNGQTDAAVGAAGLAVLHGYSGAVDQHGNELLVTEVAIADEIAAAADLVKGKLTAMPVAVVRGLSVTDDGSTARQLLRPGTEDLFWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPAELVREAVAEALTAPAPHHTRPVRFVWLQTPAVRTRLLDAMKDKWRADLAGDGRPAESIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVGLAVRGLGSCWIGSTIFAADLVRAVLGLPADWEPLGAIAIGYAAEPAGPRDPADPGDLLIRK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000479
EMBL· GenBank· DDBJ
ABK64439.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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