A0Q4N6 · LPXF_FRATN

Function

function

Removes the 4'-phosphate moiety from lipid IV(A) (a tetraacylated precursor of lipid A) and from pentaacylated lipid A, but not from hexaacylated lipid A (as is found in E.coli). Does not dephosphorylate phosphatidic acid, phosphatidylglycerophosphate, or the 1-phosphate group of lipid A and lipid A precursors. Its expression in E.coli confers resistance to the cationic antimicrobial peptide (CAMP) polymyxin B (PubMed:16467300).
Plays a critical role in the ability of the bacteria to avoid the host's innate immune system, especially the bactericidal action of CAMPs, although whether it is CAMP-sensitivity or increased sensitivity to the immune system is not clear (PubMed:17360489).

Miscellaneous

In Francisella lipid A is mono-phosphorylated at position 1- and tetraacylated, it lacks the 4'-phosphate residue found in E.coli (PubMed:17263332, PubMed:17360489).
More than 90% of lipid A is in a free form that lacks core oligosaccharide and O-antigen (PubMed:17360489).

Pathway

Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionhydrolase activity
Biological Processlipid A biosynthetic process
Biological Processlipopolysaccharide biosynthetic process
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lipid A 4'-phosphatase
  • EC number

Gene names

    • Name
      lpxF
    • ORF names
      AW25_1746
    • Ordered locus names
      FTN_0295

Organism names

Accessions

  • Primary accession
    A0Q4N6
  • Secondary accessions
    • Q1WDN3

Proteomes

Subcellular Location

Cell inner membrane
; Multi-pass membrane protein
Note: Activity depends on msbA function when expressed in E.coli, strongly suggesting this protein acts in the periplasm (MsbA flips the lipopolysaccharide precursor across the cell inner membrane).

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-3Cytoplasmic
Transmembrane4-24Helical; Name=1
Topological domain25-58Periplasmic
Transmembrane59-79Helical; Name=2
Topological domain80-87Cytoplasmic
Transmembrane88-108Helical; Name=3
Topological domain109-144Periplasmic
Transmembrane145-165Helical; Name=4
Topological domain166-169Cytoplasmic
Transmembrane170-190Helical; Name=5
Topological domain191-193Periplasmic
Transmembrane194-214Helical; Name=6
Topological domain215-222Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Lipid A retains the 4'-phosphate group (giving bi-phosphorylated lipid A) and a 3'-hydroxyacyl chain not usually seen in wild-type cells. Cells grow more slowly in liquid culture and are more sensitive to the CAMP polymyxin B. Bacteria are avirulent upon infection of C57BL/6 mice, the natural host of this bacterium; they do not colonize mouse spleen. Upon mouse intraperitoneal injection induces pro-inflammatory cytokine IL-6 and monocyte chemoattractant protein 1 and keratinocyte-derived chemokine, two potent chemoattractants; bacterial viability decreases about 100-fold.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004324951-222Lipid A 4'-phosphatase

Family & Domains

Sequence similarities

Belongs to the lipid A LpxF 4'-phosphatase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    222
  • Mass (Da)
    25,552
  • Last updated
    2007-01-09 v1
  • Checksum
    15B8850E5BD330E3
MARFHIILGLVVCFFAWIFFLIFPNLDIQFAGHFYNSSAHQFIGGYDGFLGFLHWFARFFPIFFSIIVILFLLGSLFIDKFKIKYRKAIFFIAVCLWIGPGLVVNYVFKDHWGRPRPVMVEQFNGDKIFQPPFVISSQCDKNCSFVCGDASMGFWLFAFMPLLATRKKKLVAFIAAVVAGGGLGLMRMSQGGHFFSDVVFCGIFVYISTWVVYALMYRKKEY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ364143
EMBL· GenBank· DDBJ
ABC96319.1
EMBL· GenBank· DDBJ
Genomic DNA
CP000439
EMBL· GenBank· DDBJ
ABK89201.1
EMBL· GenBank· DDBJ
Genomic DNA
CP009633
EMBL· GenBank· DDBJ
AJI61280.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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