A0NXQ9 · T3HPD_ROSAI
- ProteinTrans-3-hydroxy-L-proline dehydratase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids341 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the dehydration of trans-3-hydroxy-L-proline (t3LHyp) to Delta1-pyrroline-2-carboxylate (Pyr2C). May be involved in a degradation pathway of t3LHyp, which would allow L.aggregata to grow on t3LHyp as a sole carbon source. Displays neither proline racemase activity nor 4-hydroxyproline 2-epimerase activity.
Catalytic activity
- trans-3-hydroxy-L-proline = 1-pyrroline-2-carboxylate + H2O
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
7.8 mM | trans-3-hydroxy-L-proline |
kcat is 15 sec-1 for t3LHyp dehydration.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 90 | Proton acceptor | |||
Binding site | 91-92 | substrate | |||
Binding site | 252 | substrate | |||
Binding site | 257-258 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4-hydroxyproline epimerase activity | |
Molecular Function | trans-L-3-hydroxyproline dehydratase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTrans-3-hydroxy-L-proline dehydratase
- EC number
- Short namesT3LHyp dehydratase; t3HypD
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Stappiaceae > Roseibium
Accessions
- Primary accessionA0NXQ9
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000432271 | 1-341 | Trans-3-hydroxy-L-proline dehydratase | ||
Expression
Induction
Is up-regulated when the bacterium is grown on t4LHyp or t3LHyp as sole carbon source.
Structure
Sequence
- Sequence statusComplete
- Length341
- Mass (Da)36,291
- Last updated2007-01-09 v1
- Checksum168E2D45B5E3C8B9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAUW01000014 EMBL· GenBank· DDBJ | EAV42586.1 EMBL· GenBank· DDBJ | Genomic DNA |