A0NXQ9 · T3HPD_ROSAI

Function

function

Catalyzes the dehydration of trans-3-hydroxy-L-proline (t3LHyp) to Delta1-pyrroline-2-carboxylate (Pyr2C). May be involved in a degradation pathway of t3LHyp, which would allow L.aggregata to grow on t3LHyp as a sole carbon source. Displays neither proline racemase activity nor 4-hydroxyproline 2-epimerase activity.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
7.8 mMtrans-3-hydroxy-L-proline
kcat is 15 sec-1 for t3LHyp dehydration.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site90Proton acceptor
Binding site91-92substrate
Binding site252substrate
Binding site257-258substrate

GO annotations

AspectTerm
Molecular Function4-hydroxyproline epimerase activity
Molecular Functiontrans-L-3-hydroxyproline dehydratase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Trans-3-hydroxy-L-proline dehydratase
  • EC number
  • Short names
    T3LHyp dehydratase; t3HypD
  • Alternative names
    • Trans-L-3-hydroxyproline dehydratase

Gene names

    • ORF names
      SIAM614_28502

Organism names

Accessions

  • Primary accession
    A0NXQ9

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004322711-341Trans-3-hydroxy-L-proline dehydratase

Expression

Induction

Is up-regulated when the bacterium is grown on t4LHyp or t3LHyp as sole carbon source.

Structure

Family & Domains

Sequence similarities

Belongs to the proline racemase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    341
  • Mass (Da)
    36,291
  • Last updated
    2007-01-09 v1
  • Checksum
    168E2D45B5E3C8B9
MRSTKTIHVISCHAEGEVGDVIVGGVAPPPGETLWEQRSFIARDQTLRNFVLNEPRGGVFRHVNLLVPPRHPEADAAFIIMEPEDTPPMSGSNSICVSTVLLDGGIVPMIEPITEMVLEAPGGLVRVKAECRNGKAERIFVQNVTSFADKLSVPLDVEGIGTLTVDTAYGGDSFVVVDAEALGFAIVEDEAKDIARLGVRITNAANEQLGFSHPENPDWNHISFCAFCGPLSQTPTGLTGRSAVAIQPGKVDRSPTGTAVSARMALMAARGQMTIGDTFEAVSIIGSSFTGRIVSQQMAGDRPGIVPEISGRGWITGIHQHMLDPSDPWPGGYKLSDTWGA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAUW01000014
EMBL· GenBank· DDBJ
EAV42586.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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