A0MGZ7 · H6S3B_DANRE
- ProteinHeparan-sulfate 6-O-sulfotransferase 3-B
- Genehs6st3b
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids419 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.
Catalytic activity
- 3'-phosphoadenylyl sulfate + alpha-D-glucosaminyl-[heparan sulfate](n) = 6-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H+
CHEBI:58339 + α-D-glucosaminyl-[heparan sulfate](n) RHEA-COMP:9830 = 6-sulfo-α-D-glucosaminyl-[heparan sulfate](n) RHEA-COMP:14621 + CHEBI:58343 + CHEBI:15378
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 101-109 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: HIQKTGGTT | ||||||
Binding site | 131-132 | substrate | ||||
Sequence: KK | ||||||
Binding site | 148 | substrate | ||||
Sequence: R | ||||||
Binding site | 153 | substrate | ||||
Sequence: W | ||||||
Active site | 158 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 158 | substrate | ||||
Sequence: H | ||||||
Binding site | 191 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 199 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 203 | substrate | ||||
Sequence: H | ||||||
Binding site | 210 | substrate | ||||
Sequence: W | ||||||
Binding site | 323-325 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: TQI | ||||||
Binding site | 329-330 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: RA |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | 3'-phosphoadenosine 5'-phosphosulfate binding | |
Molecular Function | heparan sulfate 6-O-sulfotransferase activity | |
Molecular Function | oligosaccharide binding | |
Molecular Function | sulfotransferase activity | |
Biological Process | heparan sulfate proteoglycan biosynthetic process, enzymatic modification |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHeparan-sulfate 6-O-sulfotransferase 3-B
- EC number
- Short namesHS 6-OST-3-B
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionA0MGZ7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-7 | Cytoplasmic | ||||
Sequence: MNDKPNK | ||||||
Transmembrane | 8-28 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: WIFIPILAILFVMIGYQYVCP | ||||||
Topological domain | 29-419 | Lumenal | ||||
Sequence: AGGQACHFRTGDKLVRIAPLATPDPTTDDLYREQDPEEDNPPKCASKFNFTERDLTRDVDFNIKGDDVIVFLHIQKTGGTTFGRHLVRNIRLEQPCDCKAGQKKCTCHRPGKQESWLFSRFSTGWSCGLHADWTELTNCVPVIMDKRQPPKRKRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTQDELPTCYNGDDWSGVTLHDFMDCPSNLANNRQVRMLADLSLVGCYNLSTMNESERNPILLASAKSNLKNMAFYGLTEFQRKTQYLFERTFHLRFISAFTQINSTRAANVELRDDMRSRIEQLNMLDMQLYEFAKDLFLQRYQFVRQRERQEERLKRREERRWIRERRVNQSKEPIVENQTRVTTTEDYASQVVRW |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 101 | Significantly impairs catalytic activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 104 | Significantly impairs catalytic activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 131 | Slightly impairs catalytic activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 132 | Significantly impairs catalytic activity. | ||||
Sequence: K → A or E | ||||||
Mutagenesis | 148 | Moderately impairs catalytic activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 153 | Moderately impairs catalytic activity. | ||||
Sequence: W → A | ||||||
Mutagenesis | 158 | Abolishes catalytic activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 203 | Significantly impairs catalytic activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 210 | Significantly impairs catalytic activity. | ||||
Sequence: W → A |
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000283820 | 1-419 | Heparan-sulfate 6-O-sulfotransferase 3-B | |||
Sequence: MNDKPNKWIFIPILAILFVMIGYQYVCPAGGQACHFRTGDKLVRIAPLATPDPTTDDLYREQDPEEDNPPKCASKFNFTERDLTRDVDFNIKGDDVIVFLHIQKTGGTTFGRHLVRNIRLEQPCDCKAGQKKCTCHRPGKQESWLFSRFSTGWSCGLHADWTELTNCVPVIMDKRQPPKRKRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTQDELPTCYNGDDWSGVTLHDFMDCPSNLANNRQVRMLADLSLVGCYNLSTMNESERNPILLASAKSNLKNMAFYGLTEFQRKTQYLFERTFHLRFISAFTQINSTRAANVELRDDMRSRIEQLNMLDMQLYEFAKDLFLQRYQFVRQRERQEERLKRREERRWIRERRVNQSKEPIVENQTRVTTTEDYASQVVRW | ||||||
Glycosylation | 77 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 270 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 275 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 326 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 393 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 402 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
In early somitogenesis, expressed in presumptive forebrain and midbrain, tail bud and Kupffer's vesicle. During mid-somitogenesis, ubiquitous expression which is strongest in the somites and eye. During late somitogenesis, predominantly expressed in eye, hindbrain and ventral somites. At 24 hours post-fertilization (hpf), restricted to lens and neural retina, brain, otic vesicle and somites. At 36 hpf, brain expression is restricted to telencephalon. At 48 hpf, restricted to telencephalon and pectoral fin.
Developmental stage
Expressed both maternally and zygotically.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the sulfotransferase 6 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length419
- Mass (Da)49,346
- Last updated2006-12-12 v1
- ChecksumD83CDCF7356749B5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ812996 EMBL· GenBank· DDBJ | ABH11458.1 EMBL· GenBank· DDBJ | mRNA |