A0JJT9 · TENB_BEABA
- ProteinCytochrome P450 monooxygenase tenB
- GenetenB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids539 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of tenellin-type 2-pyridones, iron-chelating compounds involved in iron stress tolerance, competition with the natural competitor fungus Metarhizium robertsii and insect hosts infection (PubMed:17216664, PubMed:19067514, PubMed:20575135, PubMed:34903054).
TenB catalyzes the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively (PubMed:19067514, PubMed:34903054).
The pathway begins with the assembly of the polyketide-amino acid backbone by the hybrid PKS-NRPS tenS with the help of the enoyl reductase tenC. These enzymes catalyze the synthesis of the pyrrolidine-2-dione intermediates pretellinin A, 11-hydropretellenin A, 12-hydropretellenin A, 13-hydropretellenin A, 14-hydropretellenin A, 12-oxopretellenin A and prototellinin D. The cytochrome P450 monooxygenase tenA then catalyzes an oxidative ring expansion of pretenellin A and 14-hydropretellenin A to form the 2-pyridone core, leading to pretenellin B and pyridovericin, respectively. The cytochrome P450 monooxygenase tenB is then required for the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively. The UDP-glucosyltransferase GT1 and the methyltransferase MT1, located outside the tenS gene cluster, contribute to the stepwise glycosylation and methylation of 15-HT to obtain the glycoside pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP). Additional related compounds such as 1-O-methyl-15-HT, (8Z)-1-O-methyl-15-HT, and O-methyltenellin A are also produced but the enzymes involved in their biosynthesis have still to be determined (PubMed:34903054).
TenB catalyzes the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively (PubMed:19067514, PubMed:34903054).
The pathway begins with the assembly of the polyketide-amino acid backbone by the hybrid PKS-NRPS tenS with the help of the enoyl reductase tenC. These enzymes catalyze the synthesis of the pyrrolidine-2-dione intermediates pretellinin A, 11-hydropretellenin A, 12-hydropretellenin A, 13-hydropretellenin A, 14-hydropretellenin A, 12-oxopretellenin A and prototellinin D. The cytochrome P450 monooxygenase tenA then catalyzes an oxidative ring expansion of pretenellin A and 14-hydropretellenin A to form the 2-pyridone core, leading to pretenellin B and pyridovericin, respectively. The cytochrome P450 monooxygenase tenB is then required for the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively. The UDP-glucosyltransferase GT1 and the methyltransferase MT1, located outside the tenS gene cluster, contribute to the stepwise glycosylation and methylation of 15-HT to obtain the glycoside pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP). Additional related compounds such as 1-O-methyl-15-HT, (8Z)-1-O-methyl-15-HT, and O-methyltenellin A are also produced but the enzymes involved in their biosynthesis have still to be determined (PubMed:34903054).
Cofactor
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 monooxygenase tenB
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Cordycipitaceae > Beauveria
Accessions
- Primary accessionA0JJT9
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 13-33 | Helical | ||||
Sequence: LGYYEKVAGVLGFLSIALLFW |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000438452 | 1-539 | Cytochrome P450 monooxygenase tenB | |||
Sequence: MALFQAMSMVAQLGYYEKVAGVLGFLSIALLFWKLNHKPFYPALPLAGEPPQRRWFSLSNRLRYYNDCAALFDEAYHTAYAKKGKAVLVPSMGVHTAMIMPESAMNWAMSQPDDSLSIKKAFSELNQTKYSLGHGRYWEDPWQLDLVKAHLSSILQNLIPQLNEELAAAFSKHLGTDAENWKEIELEVIMRRIIAQATSRFIVGLPLCRDDGYLDLSYKVILGMVTTIWATLPFPDLIRAITGPIASWQTRRNIARIQEYLEPLYQERISILESRDGPESDPEPQDLFMMMLRFAQKKRPDEYANLGIMTRRVCAANFVAMHQSTVSVTNLILNIIGSDAEFNTTATLRDEITQVMRGTDAKSWTKDTFTRMRKCDSVAREAMRLNFPLGTRGSMRAVLKDGLESPEGIKLQKGTTISWLASCAQVDADRFDNPQKFDPFRFSRASKDDDDDGKSTSSHAKDAFVTTSPQYLPFGHGKHACPGRFMVDLMFKILLAQLLTHYDLGWPEDYQGKQPPSVWQGELSEPPPGARILVKRRKV |
Expression
Induction
Expression is positively regulated by the cluster-specific transcription factor tenR and is induced during cocultures with the natural competitor fungus Metarhizium robertsii.
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 439-458 | Basic and acidic residues | ||||
Sequence: PFRFSRASKDDDDDGKSTSS | ||||||
Region | 439-460 | Disordered | ||||
Sequence: PFRFSRASKDDDDDGKSTSSHA |
Sequence similarities
Belongs to the cytochrome P450 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length539
- Mass (Da)61,065
- Last updated2006-12-12 v1
- ChecksumFDCF0430D15A2C82
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 439-458 | Basic and acidic residues | ||||
Sequence: PFRFSRASKDDDDDGKSTSS |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HM243222 EMBL· GenBank· DDBJ | ADN43683.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AM409327 EMBL· GenBank· DDBJ | CAL69595.1 EMBL· GenBank· DDBJ | Genomic DNA |