A0AAN3XEN5 · A0AAN3XEN5_LACRH
- ProteinCCA-adding enzyme
- Genecca
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids398 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.
Miscellaneous
A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
Catalytic activity
- a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate
- a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 32 | ATP (UniProtKB | ChEBI) | |||
Binding site | 32 | CTP (UniProtKB | ChEBI) | |||
Binding site | 35 | ATP (UniProtKB | ChEBI) | |||
Binding site | 35 | CTP (UniProtKB | ChEBI) | |||
Binding site | 45 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 47 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 116 | ATP (UniProtKB | ChEBI) | |||
Binding site | 116 | CTP (UniProtKB | ChEBI) | |||
Binding site | 159 | ATP (UniProtKB | ChEBI) | |||
Binding site | 159 | CTP (UniProtKB | ChEBI) | |||
Binding site | 162 | ATP (UniProtKB | ChEBI) | |||
Binding site | 162 | CTP (UniProtKB | ChEBI) | |||
Binding site | 165 | ATP (UniProtKB | ChEBI) | |||
Binding site | 165 | CTP (UniProtKB | ChEBI) | |||
Binding site | 168 | ATP (UniProtKB | ChEBI) | |||
Binding site | 168 | CTP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | nucleotidyltransferase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCCA-adding enzyme
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Lacticaseibacillus
Accessions
- Primary accessionA0AAN3XEN5
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 27-147 | Poly A polymerase head | |||
Domain | 174-233 | tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding | |||
Domain | 250-389 | CCA-adding enzyme C-terminal | |||
Sequence similarities
Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length398
- Mass (Da)43,977
- Last updated2024-10-02 v1
- ChecksumE43883D905C05E7B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AMQW01000004 EMBL· GenBank· DDBJ | EKS52489.1 EMBL· GenBank· DDBJ | Genomic DNA |