A0AAN3XDH1 · A0AAN3XDH1_LACRH
- ProteinLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT
- GenemurT
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids450 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.
Catalytic activity
- beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamine + ATP + H2O = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamate + ADP + phosphate + H+
- beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + ATP = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + ADP
- beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4+ = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + phosphate + H+
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 208 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 211 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 230 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 233 | Zn2+ (UniProtKB | ChEBI) | |||
Active site | 358 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ligase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Lacticaseibacillus
Accessions
- Primary accessionA0AAN3XDH1
Proteomes
Interaction
Subunit
Forms a heterodimer with GatD.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 56-189 | Mur ligase central | |||
Domain | 322-433 | Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT C-terminal | |||
Sequence similarities
Belongs to the MurCDEF family. MurT subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length450
- Mass (Da)49,409
- Last updated2024-10-02 v1
- MD5 Checksum156FA0E0CE22B293221725CB16E57B89
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AMQW01000007 EMBL· GenBank· DDBJ | EKS51273.1 EMBL· GenBank· DDBJ | Genomic DNA |