A0AAN1B2V6 · A0AAN1B2V6_MYCSY

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

169750100150200250300350400450500550600650
Type
IDPosition(s)Description
Binding site34-38NAD+ (UniProtKB | ChEBI)
Binding site83-84NAD+ (UniProtKB | ChEBI)
Binding site114NAD+ (UniProtKB | ChEBI)
Active site116N6-AMP-lysine intermediate
Binding site137NAD+ (UniProtKB | ChEBI)
Binding site171NAD+ (UniProtKB | ChEBI)
Binding site315NAD+ (UniProtKB | ChEBI)
Binding site339NAD+ (UniProtKB | ChEBI)
Binding site430Zn2+ (UniProtKB | ChEBI)
Binding site433Zn2+ (UniProtKB | ChEBI)
Binding site448Zn2+ (UniProtKB | ChEBI)
Binding site453Zn2+ (UniProtKB | ChEBI)

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      OIE46_03100

Organism names

Accessions

  • Primary accession
    A0AAN1B2V6

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain616-697BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    697
  • Mass (Da)
    80,491
  • Last updated
    2024-10-02 v1
  • Checksum
    1CA9EC2F644429E6
MSNQQIKEKIISLNNYLKHLNHLYYDLDAPEVDDKTYDSLYSELLELEAKYPNLVLEDSVTKIIGAFVSNKFKKTKHNKEMLSLDKAYKKSEIFSFYENFTQYKNLENFGFSLEPKIDGLSISIHYDNGKFIKAITRGDGTTGEDVSENVFQIRDVPKQISYLKPLEVRGEIYMKKSTWKSLNEDIKNQYFQTNIEKIFSYMQALPKSKSWSNLKIKNPIFFKNARNAAAGTLRQKNAKIVKSRNLSSLFYEIVSPLEHNLKTQMEVLAFLKEQNFEVNEFQKLVKNDQEIMFEINEFSKIKNNFEFDCDGFVIKFNLIDKWEQIGFTSKFPKWAIAYKYMLEEANTKILNIVAQVGRTGNITYIAQFMPVELNNTTVQNATLHNYEFIKKNNINIGDEITIIKSGEIIPKVISIYKKNTDSVFEKVLNCPSCNSLLEIPEGYVDQFCRNENCDEKKIQMLTFFVSKNCLNITNLSVQNIRIFYNHPVIQMREIQDIFLLKNHVDEIKKIKFFAGKSQENKKINNILQSIEKAKDAYLRNVLAALGIKGIGNIAANLLTNKITKLSDLNNLTDEDLLSIDTFGEKSIENLKEFLSSEKNQELIKFLDENLNYLNSKKSSKLNNLNFAITGSLSISRDEFKKIILDNGGIFSNSVSKNTSYLISNSKENSTKIKKALENNIKVITEEEFHNLLKEENA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP107525
EMBL· GenBank· DDBJ
UZW64331.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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