A0AAJ7BKR4 · A0AAJ7BKR4_CEPCN
- ProteinHedgehog protein
- GeneLOC107264577
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids457 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product. Once cleaved, the C-product has no signaling activity and diffuses from the cell.
Protein hedgehog N-product
The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development.
Protein hedgehog
The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product.
Catalytic activity
- glycyl-L-cysteinyl-[protein] + cholesterol + H+ = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein]This reaction proceeds in the forward direction.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 103 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 104 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 104 | Ca2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 109 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 139 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 140 | Ca2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 140 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 143 | Ca2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 154 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 161 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 196 | Zn2+ (UniProtKB | ChEBI) | |||
Site | 211-212 | Cleavage; by autolysis | |||
Site | 257 | Involved in cholesterol transfer | |||
Site | 280 | Involved in auto-cleavage | |||
Site | 283 | Essential for auto-cleavage | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | extracellular space | |
Cellular Component | Golgi membrane | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Molecular Function | calcium ion binding | |
Molecular Function | morphogen activity | |
Molecular Function | patched binding | |
Molecular Function | peptidase activity | |
Molecular Function | transferase activity | |
Biological Process | anatomical structure morphogenesis | |
Biological Process | cell fate specification | |
Biological Process | cell-cell signaling | |
Biological Process | intein-mediated protein splicing | |
Biological Process | protein autoprocessing | |
Biological Process | regulation of gene expression | |
Biological Process | segment polarity determination | |
Biological Process | smoothened signaling pathway | |
Biological Process | system development |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameHedgehog protein
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Hymenoptera > Cephoidea > Cephidae > Cephus
Accessions
- Primary accessionA0AAJ7BKR4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Sonic hedgehog protein
Protein hedgehog N-product
Cell membrane ; Lipid-anchor
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-31 | ||||
Chain | PRO_5042578158 | 32-457 | Hedgehog protein | ||
Keywords
- PTM
Interaction
Subunit
Interacts with shf.
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 200-220 | Polar residues | |||
Region | 200-226 | Disordered | |||
Domain | 210-308 | Hint | |||
Domain | 332-376 | Hint | |||
Sequence similarities
Belongs to the hedgehog family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length457
- Mass (Da)51,145
- Last updated2024-07-24 v1
- ChecksumE52661CA894B85B7
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 200-220 | Polar residues | |||
Keywords
- Technical term