A0AAJ2U3Z3 · A0AAJ2U3Z3_ALKPS
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids668 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 34-38 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: DAEYD | ||||||
Binding site | 83-84 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: SL | ||||||
Binding site | 113 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 115 | N6-AMP-lysine intermediate | ||||
Sequence: K | ||||||
Binding site | 136 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 170 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 286 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 310 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 404 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 407 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 422 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 427 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ligase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Alkalihalophilus
Accessions
- Primary accessionA0AAJ2U3Z3
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 591-668 | BRCT | ||||
Sequence: AAESPFANKTVVLTGKLYEMTRDEAKEKIETLGGKVTGSVSKKTDIVIAGEDAGSKKKKAEELGIEIWDEAALLKGLT |
Sequence similarities
Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length668
- Mass (Da)74,173
- Last updated2024-07-24 v1
- Checksum98BCD5F061853E88