A0AAJ2U3Z3 · A0AAJ2U3Z3_ALKPS

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site34-38NAD+ (UniProtKB | ChEBI)
Binding site83-84NAD+ (UniProtKB | ChEBI)
Binding site113NAD+ (UniProtKB | ChEBI)
Active site115N6-AMP-lysine intermediate
Binding site136NAD+ (UniProtKB | ChEBI)
Binding site170NAD+ (UniProtKB | ChEBI)
Binding site286NAD+ (UniProtKB | ChEBI)
Binding site310NAD+ (UniProtKB | ChEBI)
Binding site404Zn2+ (UniProtKB | ChEBI)
Binding site407Zn2+ (UniProtKB | ChEBI)
Binding site422Zn2+ (UniProtKB | ChEBI)
Binding site427Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionligase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      RYX45_16210

Organism names

Accessions

  • Primary accession
    A0AAJ2U3Z3

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain591-668BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    668
  • Mass (Da)
    74,173
  • Last updated
    2024-07-24 v1
  • Checksum
    98BCD5F061853E88
MDRQEAEKRVQTLREKLEEYGYHYYVLDQPLVTDAEYDQLMQELIALEGEHPELVSEDSPSVRVGGEPLPFFVKVEHQIPMLSLGNAFNDQDLRDFDRRVRERVGDNVTYSCELKIDGLAVSLTYENGKFVRGATRGDGTIGEDITNNLKTIPAIPLKLKQEASLEVRGEAYMPKKSFEALNEAKSEAGEEKFANPRNAAAGSLRQLDPKIAAKRNLSIFIYAIGNAEGQDLASHHEGLAYLKELGFKVNNESKHCPTIEEVIEYTNEWLDKRADLPYEIDGIVIKVDSIEQQKELGFTAKSPRWATAFKFPAEEVVTVLRDIELSVGRTGVVTPTAILDPVVVAGTTVKRASLHNEDLIREKDVKLGDTVIVKKAGDIIPEVVNVLTDRRDGRERDFSMPTHCPECESELVRLEGEVALRCINPECPAQIREGLIHFVSRQAMNIDGLGEKVIAQLFDHKLIHTVADLYQLDRSELLKLERMGEKSVDNLLKAIEASKENSLEKLLFGLGIRFVGSKAAKTLAMEFESIEAIIEADEAALVAVNEIGEKMAASIVRFFETDEVKELIKRLQSYGVNTSYKGPKPVSGTEAAESPFANKTVVLTGKLYEMTRDEAKEKIETLGGKVTGSVSKKTDIVIAGEDAGSKKKKAEELGIEIWDEAALLKGLT

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAWJAY010000004
EMBL· GenBank· DDBJ
MDV2886737.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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