A0AAJ1XMI3 · A0AAJ1XMI3_9STAP

  • Protein
    ATP-dependent 6-phosphofructokinase
  • Gene
    pfkA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site11ATP (UniProtKB | ChEBI)
Binding site21-25ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners
Binding site72-73ATP (UniProtKB | ChEBI)
Binding site102-105ATP (UniProtKB | ChEBI)
Binding site103Mg2+ (UniProtKB | ChEBI); catalytic
Binding site127-129substrate; ligand shared between dimeric partners; in other chain
Active site129Proton acceptor
Binding site156ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site164substrate; ligand shared between dimeric partners
Binding site171-173substrate; ligand shared between dimeric partners; in other chain
Binding site187-189ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site213ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site215-217ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site224substrate; ligand shared between dimeric partners; in other chain
Binding site245substrate; ligand shared between dimeric partners
Binding site251-254substrate; ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Molecular Functiontransferase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent 6-phosphofructokinase
  • EC number
  • Short names
    ATP-PFK
    ; Phosphofructokinase
  • Alternative names
    • Phosphohexokinase

Gene names

    • Name
      pfkA
    • ORF names
      RG670_05170

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • FR124
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus

Accessions

  • Primary accession
    A0AAJ1XMI3

Proteomes

Subcellular Location

Cytoplasm

Keywords

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain3-277Phosphofructokinase

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    322
  • Mass (Da)
    34,596
  • Last updated
    2024-07-24 v1
  • Checksum
    9609BFF232C81388
MKKIAVLTSGGDSPGMNAAVRAVVRKAIYNNIEVYGIYQGYQGLLDDNIHKLELGSVGDTIQRGGTFLYSARCPQFKEASVRQKGIENLHKRGIEGLVVVGGDGSYRGAQRISEETKDIKTIGIPGTIDNDINGTDFTIGFDTALNTIIDSVDKIRDTASSHARTFIVEVMGRDCGDLALWAGLSVGAETILVPEANTDIKDIAEKIESGIKRGKKHSIVMVAEGSMSGQECADQLMKYINVDARVSVLGHIQRGGSPSGADRVLASRLGGYAVDLLMQGRSGQGVGIMNNKLTATPFDEIFNGNDHKFNTDIYELAKELSI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAKZIJ010000013
EMBL· GenBank· DDBJ
MDQ8622871.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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