A0AAI9AH98 · A0AAI9AH98_9BACT

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site117O-(5'-phospho-DNA)-tyrosine intermediate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA
    • ORF names
      CMTB2_01633

Organism names

Accessions

  • Primary accession
    A0AAI9AH98

Proteomes

Subcellular Location

Cytoplasm

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Family & Domains

Features

Showing features for domain, coiled coil, motif.

TypeIDPosition(s)Description
Domain6-458DNA topoisomerase type IIA
Coiled coil423-450
Motif519-525GyrA-box

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    812
  • Mass (Da)
    91,698
  • Last updated
    2024-07-24 v1
  • Checksum
    0E6EB70712B9727C
MQVIPVKIEDTLAKSYLEYSMSVIVGRALPDVRDGLKPVHRRILYAMYKMGITSSSPYKKSARIVGDVIGKYHPHGDTAVYEALVRMAQNFSMREPLIDGQGNFGSIDGDNAAAMRYTEARLTKIAEELLKDIDKDTVDFIPNYDGTEKEPVVLPARFPNLLVNGSSGIAVGMATNIPPHNLGELIDALVYMIDNKETNLEDILQFIKGPDFPTGGIIFGRGGILEAYKSGRGSIKIRAKHHIEHRGNREIIVIDELPYQVNKAKLIEKISELVKDKIIDGISEIRDESDREGIRVVIELKKDAMSEIILNNLYKHTQMQVSFGINMLAILDKEPKLFNLMEILNTFIKFRKTVVIRRTIYELEEAKKRAHILEGLLIALANIDEVVTIIKNSQSSKEAKTNLIERFDLSEVQAQAILDMKLSRLTSLETNKLQKEYDELQEKIAYLTSILKEESVLNNVIKEEFLEIRKKYANERKTEIVDDYDDIDIEDLIPNEDMVVTITHRGYVKRVPLKTYERQNRGGKGKKALTTYENDFIEDFYIAKAHDTLMIITNKGQLHWLKVYKIPEGSRTSKGKAIVNLINLDKDEKIQTIIKTSDFDENKSLAFFTKNGIVKRTNLSEFKNIRSTGVRAIVIDKDDELVTAKIVKEDDKYLFIVTKKGMAIRFPVDAVREMGRNARGVKGITFKIEGDEVVGALSLRDEKQEILSVSEKGFGKRTEAEAYRLTNRGGKGVIAMKLTNKTGDLVGVVATEENHDLMLLTSKGKMIRVSIDSISKTGKNTQGVRLVRLDDDDKVVSVAKTPSQKEELLIEE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ABCJ01000001
EMBL· GenBank· DDBJ
EDM24176.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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