A0AAI8MIU5 · A0AAI8MIU5_9BRAD

  • Protein
    Carbamoyl phosphate synthase small chain
  • Gene
    carA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site59L-glutamine (UniProtKB | ChEBI)
Binding site256L-glutamine (UniProtKB | ChEBI)
Binding site258L-glutamine (UniProtKB | ChEBI)
Active site285Nucleophile
Binding site286L-glutamine (UniProtKB | ChEBI)
Binding site289L-glutamine (UniProtKB | ChEBI)
Binding site327L-glutamine (UniProtKB | ChEBI)
Binding site329L-glutamine (UniProtKB | ChEBI)
Binding site330L-glutamine (UniProtKB | ChEBI)
Active site369
Active site371

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process
Biological Processpyrimidine nucleotide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      S23_59720

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • S23321
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Bradyrhizobium

Accessions

  • Primary accession
    A0AAI8MIU5

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-199CPSase
Domain15-149Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    396
  • Mass (Da)
    42,746
  • Last updated
    2024-07-24 v1
  • Checksum
    22052286C82A832C
MTQHENDTAWPDHKPTALLVLADGTVLEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRTAITDPSNYRATKHLDAWLKARGIIGLSGIDTRALTALIRSKGMPNAVIVHSRTGEFDLHGLKEEAREWPGLEGMDLVPMVTSGQRFTWDETPWLWDKGFGRQDKPEFNVVAIDYGIKRNILRLLAGVGCKVTVVPATTSSEEILAMKPDGVFLSNGPGDPAATGKYAVPVIQDVIKSGTPTFGICLGHQMLGLAVGAKTKKMHQGHHGANHPVKDETTGKVEITSMNHGFAVDENTLPKGATQTHISLFDGSNCGIQLDGKPVFSVQYHPEASPGPRDSHYLFQRFADLMRQKKSA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012279
EMBL· GenBank· DDBJ
BAL79161.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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