A0AAI8MHZ4 · A0AAI8MHZ4_9BRAD

  • Protein
    7-carboxy-7-deazaguanine synthase
  • Gene
    queE
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

S-adenosyl-L-methionine (UniProtKB | Rhea| CHEBI:59789 )

Note: Binds 1 S-adenosyl-L-methionine per subunit.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site12-14substrate
Binding site27substrate
Binding site31[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site46[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site48-50S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site49[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site51Mg2+ (UniProtKB | ChEBI)
Binding site90substrate
Binding site92S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site133-135S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site173-176S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-nitrogen lyase activity
Molecular Functionmagnesium ion binding
Molecular FunctionS-adenosyl-L-methionine binding
Biological Processqueuosine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    7-carboxy-7-deazaguanine synthase
  • EC number
  • Short names
    CDG synthase
  • Alternative names
    • Queuosine biosynthesis protein QueE

Gene names

    • Name
      queE
    • ORF names
      S23_55040

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • S23321
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Bradyrhizobium

Accessions

  • Primary accession
    A0AAI8MHZ4

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain18-210Radical SAM core

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    210
  • Mass (Da)
    22,595
  • Last updated
    2024-07-24 v1
  • Checksum
    C94F306B0E16FF7B
MSYAVKEIFLTLQGEGAHAGRASVFCRFAGCNLWSGREADRLEATCKFCDTDFVGTDGTLGGRYASAAELADTIAAQWTAGGDNRYVVLTGGEPLLQVDAALVDALHARGFEIGVETNGTIAPPEGLDWICVSPKGGSELVLRHGHELKLVYPQTLAAPEAFEGLAFERFSLQPMDGPEAAQNTALAIDYCLRHPQWRLSVQTHKSLGIR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012279
EMBL· GenBank· DDBJ
BAL78696.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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