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A0AAF7AGZ0 · A0AAF7AGZ0_BOVIN

  • Protein
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • Gene
    SIRT5
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site58-77NAD+ (UniProtKB | ChEBI)
Binding site102substrate
Binding site105substrate
Binding site140-143NAD+ (UniProtKB | ChEBI)
Active site158Proton acceptor
Binding site273-275NAD+ (UniProtKB | ChEBI)
Binding site299-301NAD+ (UniProtKB | ChEBI)
Binding site317NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionNAD+ binding
Molecular FunctionNAD-dependent protein lysine deacetylase activity
Molecular Functionprotein-malonyllysine demalonylase activity
Molecular Functionprotein-succinyllysine desuccinylase activity
Molecular Functiontransferase activity
Biological Processprotein deacetylation

Keywords

  • Molecular function
  • Ligand
    • #NAD

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • EC number
  • Alternative names
    • Regulatory protein SIR2 homolog 5
    • SIR2-like protein 5

Gene names

    • Name
      SIRT5

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Hereford
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    A0AAF7AGZ0

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion
Cytoplasm, cytosol
Nucleus
Note: Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.

Keywords

Interaction

Subunit

Monomer. Homodimer. Interacts with CPS1.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain33-331Deacetylase sirtuin-type

Domain

In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity.

Sequence similarities

Belongs to the sirtuin family. Class III subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    334
  • Mass (Da)
    37,106
  • Last updated
    2024-07-24 v1
  • MD5 Checksum
    9D70D33BB66663DAB35B840BBFEAB583
MPPLWIIRNRLFSQLYCGLKSPVSTQTKICLTMARPSSNMADFRKCFAKAKHIVVISGAGISAESGVPTFRGAGGYWRKWKAQDLATPQAFARNPSQVWEFYHYRREVVQSTEPNAGHLAIAECQARLHRQGRQVVVITQNIDELHRKAGTKNLLEIHGDHPDPGTEPASPALQVAYLKLDVPLVELWLRTIRVQFVQPYQEKGNYTALWNRAPDPQTQDAGIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDKELALCDLCLVVGTSSVVYPAAMFAPQVSARGVPVAEFNMETTPATERFRFHFQGPCGTTLPEALAPHETETVS

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0AAF7AT62A0AAF7AT62_BOVINSIRT5306
A0AAA9SVU4A0AAA9SVU4_BOVINSIRT5313
A0AAA9THG1A0AAA9THG1_BOVINSIRT5202
A0AAA9S3I4A0AAA9S3I4_BOVINSIRT5253
A0AAF6Z0R7A0AAF6Z0R7_BOVINSIRT5292

Keywords

Genome annotation databases

Similar Proteins

Disclaimer

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