A0AAF0GXS7 · A0AAF0GXS7_RHIRD

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

126420406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site21dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site51(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site126-127dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site146
Active site146Nucleophile
Binding site166-169dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site169(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site177dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site207-209dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site263(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • ORF names
      CFBP5506_07800

Organism names

Accessions

  • Primary accession
    A0AAF0GXS7

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-264Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    264
  • Mass (Da)
    29,830
  • Last updated
    2024-05-29 v1
  • Checksum
    A088EB518BF06BC1
MKQYLDLLRHVMETGSDRGDRTGTGTRSVFGYQMRFDLAEGFPVLTTKKLHLRSIIHELLWFLKGDTNIAYLKENGVSIWDEWADENGDLGPVYGAQWRSWPAPDGRHIDQIALLIEALKTNPNSRRHIVSAWNPALVDEMALPPCHCLFQFYVSDGKLSCQLYQRSADIFLGVPFNIASYALLTLMVAQVVGLKPGDFVHTLGDAHIYANHFEQAKLQMTRTPKALPTMRLNPGVKDLFAFKFEDFTLENYEADSSIKAPIAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP122962
EMBL· GenBank· DDBJ
WGM58247.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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