A0AAF0B6T5 · A0AAF0B6T5_9CAUD

Function

function

Involved in countering a host defense mechanism which, following viral infection, activates the host anticodon nuclease and shuts off viral translation. Repairs 5'-PO4 and 3'-OH groups in the cleaved host tRNA.

Catalytic activity

  • ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.
    EC:6.5.1.3 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions that perform the catalytic activity via a two-metal mechanism. One of the catalytic Mg2+, which is coordinated by 5 water molecules, engages the lysine nucleophile and the ATP alpha phosphate while the Mg2+ orients the PPi leaving group.

Features

Showing features for binding site, active site, site.

137450100150200250300350
Type
IDPosition(s)Description
Binding site37ATP (UniProtKB | ChEBI)
Binding site54ATP (UniProtKB | ChEBI)
Binding site75ATP (UniProtKB | ChEBI)
Active site99N6-AMP-lysine intermediate
Binding site159ATP (UniProtKB | ChEBI)
Site159Essential for RNA ligase activity
Binding site240ATP (UniProtKB | ChEBI)
Binding site242ATP (UniProtKB | ChEBI)
Site246Essential for RNA ligase activity
Binding site272Mg2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Biological ProcessRNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA ligase 1
  • EC number
  • Alternative names
    • Rnl1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • REP3
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus

Accessions

  • Primary accession
    A0AAF0B6T5

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain52-246T4 RNA ligase 1-like N-terminal
Domain253-374T4 RNA ligase 1 C-terminal

Sequence similarities

Belongs to the Tequatrovirus RNA ligase 1 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    374
  • Mass (Da)
    43,477
  • Last updated
    2024-05-29 v1
  • Checksum
    433CAAC7A271EA80
MQELFNNLMELCKDSQRKFFYSDDVSASGRTYRIFSYNYASYSDWLLPDALECRGIMFEMDGEKPVRIASRPMEKFFNLNENPFTMNIDLNDVDYILTKEDGSLVSTYLDGDEILFKSKGSIKSEQALMANGILMNINHHQLRDRLKELAENGFTANFEFVAPTNRIVLAYQEMKIILLNIRENETGEYISYDDIYKDAVLRPYLVERYEIDSPKWVEEAKNAENIEGYVAVMKDGSHFKIKSDWYVSLHSTKSSLDNPEKLFKTIIDGASDDLKAMYADDEYSYRKIEAFETTYLKYLDRALFLVLDCHNKHCGKDRKTYAMEAQGVAKGAGMDHLFGIIMSLYQGYDSQEKVMCEIEQNFLKNYKKFIPKGY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
OQ174502
EMBL· GenBank· DDBJ
WCA45997.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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