A0AAE9WZ61 · A0AAE9WZ61_9CAUD

Function

function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, site.

1898100200300400500600700800
TypeIDPosition(s)Description
Binding site112Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site114Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site219Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site324Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site324Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site408Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site408Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site409Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site411-413substrate
Binding site479substrate
Binding site557substrate
Site618Optimization of metal coordination by the polymerase active site
Binding site620Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site620Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Site703Optimization of metal coordination by the polymerase active site
Site711Essential for viral replication

GO annotations

AspectTerm
Molecular Function3'-5' exonuclease activity
Molecular FunctionDNA binding
Molecular FunctionDNA-directed DNA polymerase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding
Biological Processbidirectional double-stranded viral DNA replication
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA-directed DNA polymerase
  • EC number

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • REP3
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus

Accessions

  • Primary accession
    A0AAE9WZ61

Proteomes

Interaction

Subunit

Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, and the primase/helicase. Interacts with the polymerase clamp; this interaction constitutes the polymerase holoenzyme.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region101-3373'-5'exonuclease
Domain106-290DNA-directed DNA polymerase family B exonuclease
Region245-261Beta hairpin
Domain365-492DNA-directed DNA polymerase family B multifunctional
Region377-898Polymerase
Domain542-627DNA-directed DNA polymerase family B multifunctional
Region702-705Binding of DNA in B-conformation
Region893-898Interaction with the polymerase clamp

Domain

The N-terminus contains the 3'-5' exonuclease activity. The C-terminus contains the polymerase activity and is involved in binding to the polymerase clamp protein. A beta hairpin structure is necessary for the proofreading function of the polymerase.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    898
  • Mass (Da)
    103,564
  • Last updated
    2024-05-29 v1
  • Checksum
    1F48C327E1BCB226
MKEFYISIETVGNNIVERYIDENGKERTREVEYLPTMFRHCKEESKYKDIYGKNCAPQKFPSMKDARDWMKRMEDIGLEALGMNDFKLAYISDTYGSEIVYDRKFVRVANCDIEVTGDKFPDPMKAEYEIDAITHYDSIDDRFYVFDLLNSMYGSVSKWDAKLAAKLDCEGGDEVPQEILDRVIYMPFDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMVLGERSMKRFSPIGRVKSKLIQNMYGSKEIYSIDGVSILDYLDLYKKFAFTNLPSFSLESVAQHETKKGKLPYDGPINKLRETNHQRYISYNIIDVESVQAIDKIRGFIDLVLSMSYYAKMPFSGVMSPIKTWDAIIFNSLKGEHKVIPQQGSHVKQSFPGAFVFEPKPIARRYIMSFDLTSLYPSIIRQVNISPETIRGQFKVHPIHEYIAGTAPKPSDEYSCSPNGWMYDKHQEGIIPKEIAKVFFQRKDWKKKMFAEEMNAEAIKKIIMKGAGSCSTKPEVERYVKFSDDFLNELSNYTESVLNSLIEECEKAATLANTNQLNRKILINSLYGALGNIHFRYYDLRNATAITIFGQVGIQWIARKINEYLNKVCGTNGEDFIAAGDTDSVYVCVDKVIEKVGLDRFKEQNDLVEFMNQFGKKKMEPMIDVAYRELCDYMNNREHLMHMDREAISCPPLGSKGVGGFWKAKKRYALNVYDMEDKRFAEPHLKIMGMETQQSSTPKAVQEALEESIRRILQEGEESVQEYYKNFEKEYRQLDYKVIAEVKTANDIAKYDDKGWPGFKCPFHIRGVLTYRRAVSGLGVAPILDGNKVMVLPLREGNPFGDKCIAWPSGTELPKEIRSDVLSWIDYSTLFQKSFVKPLAGMCESAGMDYEEKASLDFLFG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
OQ174502
EMBL· GenBank· DDBJ
WCA46095.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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