A0AAE9II95 · A0AAE9II95_9CAUD

Function

function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, site.

1898100200300400500600700800
TypeIDPosition(s)Description
Binding site112Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site114Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site219Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site324Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site324Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site408Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site408Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site409Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site411-413substrate
Binding site479substrate
Binding site557substrate
Site618Optimization of metal coordination by the polymerase active site
Binding site620Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site620Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Site703Optimization of metal coordination by the polymerase active site
Site711Essential for viral replication

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Function3'-5' exonuclease activity
Molecular FunctionDNA binding
Molecular FunctionDNA-directed DNA polymerase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding
Biological Processbidirectional double-stranded viral DNA replication
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA-directed DNA polymerase
  • EC number

Gene names

    • ORF names
      HP31_0155

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus

Accessions

  • Primary accession
    A0AAE9II95

Proteomes

Interaction

Subunit

Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, and the primase/helicase. Interacts with the polymerase clamp; this interaction constitutes the polymerase holoenzyme.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region101-3373'-5'exonuclease
Domain106-290DNA-directed DNA polymerase family B exonuclease
Region245-261Beta hairpin
Domain365-492DNA-directed DNA polymerase family B multifunctional
Region377-898Polymerase
Domain542-627DNA-directed DNA polymerase family B multifunctional
Region702-705Binding of DNA in B-conformation
Region893-898Interaction with the polymerase clamp

Domain

The N-terminus contains the 3'-5' exonuclease activity. The C-terminus contains the polymerase activity and is involved in binding to the polymerase clamp protein. A beta hairpin structure is necessary for the proofreading function of the polymerase.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    898
  • Mass (Da)
    103,592
  • Last updated
    2024-05-29 v1
  • Checksum
    0F941EC0D86B5F1B
MKEFYISIETVGNNIVERYIDENGKERTREVEYLPTMFRHCKEESKYKDIYGKNCAPQKFPSMKDARDWMKRMEDIGLEALGMNDFKLAYISDTYGSEIVYDRKFVRVANCDIEVTGDKFPDPMKAEYEIDAITHYDSIDDRFYVFDLLNSMYGSVSKWDAKLAAKLDCEGGDEVPQEILDRVIYMPFDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMVLGERSMKRFSPIGRVKSKLIQNMYGSKEIYSIDGVSILDYLDLYKKFAFTNLPSFSLESVAQHETKKGKLPYDGPINKLRETNHQRYISYNIIDVESVQAIDKIRGFIDLVLSMSYYAKMPFSGVMSPIKTWDAIIFNSLKGEHKVIPQQGSHVKQSFPGAFVFEPKPIARRYIMSFDLTSLYPSIIRQVNISPETIRGQFKVHPIHEYIAGTAPKPSEEYSCSPNGWMYDKHQEGIIPKEIAKVFFQRKDWKKKMFAEEMNAEAIKKIIMKGAGSCSTKPEVERYVKFSDDFLNELSNYTESVLNSLIEECEKAATLANTNQLNRKILINSLYGALGNIHFRYYDLRNATAITIFGQVGIQWIARKINEYLNKVCGTNGEDFIAAGDTDSVYVCVDKVIEKVGLDRFKEQNDLVEFMNQFGKKKMEPMIDVAYRELCDYMNNREHLMHMDREAISCPPLGSKGIGGFWKAKKRYALNVYDMEDKRFAEPHLKIMGMETQQSSTPKAVQEALEESIRRILQEGEESVQEYYKNFEKEYRQLDYKVIAEVKTANDIAKYDDKGWPGFKCPFHIRGVLTYRRAVSGLGVAPILDGNKVMVLPLREGNPFGDKCIAWPSGTELPKEIRSDVLSWIDYSTLFQKSFVKPLAGMCESAGMDYEEKASLDFLFG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
OK275722
EMBL· GenBank· DDBJ
URQ01283.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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