A0AAE8C5I9 · A0AAE8C5I9_9CAUD

Function

function

Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.
Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.

Catalytic activity

  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
    EC:3.5.1.28 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Zn2+ is required for amidase activity.

Activity regulation

Binding to the viral RNA polymerase inhibits amidase activity.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site19Zn2+ (UniProtKB | ChEBI)
Site48Essential for amidase activity and zinc hydrate coordination
Binding site124Zn2+ (UniProtKB | ChEBI)
Binding site132Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Molecular FunctionN-acetylmuramoyl-L-alanine amidase activity
Molecular Functionzinc ion binding
Biological Processdefense response to bacterium
Biological Processnegative regulation of viral transcription
Biological Processpeptidoglycan catabolic process
Biological Processviral release from host cell by cytolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Endolysin
  • EC number
  • Alternative names
    • N-acetylmuramoyl-L-alanine amidase

Gene names

    • ORF names
      PM139_038

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Kayfunavirus

Accessions

  • Primary accession
    A0AAE8C5I9

Proteomes

Subcellular Location

Host cytoplasm
Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.

Keywords

Expression

Keywords

Interaction

Subunit

Interacts with the viral RNA polymerase.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-128Peptidoglycan recognition protein family
Domain2-134N-acetylmuramoyl-L-alanine amidase

Sequence similarities

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    152
  • Mass (Da)
    17,103
  • Last updated
    2024-05-29 v1
  • Checksum
    1455509DF52F75CC
MVSKVQFNPRSRTDAIFVHCSATKPEMDIGVETIRMWHKQQGWLDVGYHFIIKRDGTVEEGRPVNVVGSHVKDWNSRSVGVCLVGGIDAKGKFEANFTPAQMNSLRNKLADLKDLYPQAEIKAHHDVAPKACPSFDLQRWLNTNELVTSDRG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MZ234047
EMBL· GenBank· DDBJ
QZI84511.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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