A0AAE8C5I9 · A0AAE8C5I9_9CAUD
- ProteinEndolysin
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids152 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.
Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.
Catalytic activity
Cofactor
Note: Zn2+ is required for amidase activity.
Activity regulation
Binding to the viral RNA polymerase inhibits amidase activity.
Features
Showing features for binding site, site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell cytoplasm | |
Molecular Function | N-acetylmuramoyl-L-alanine amidase activity | |
Molecular Function | zinc ion binding | |
Biological Process | defense response to bacterium | |
Biological Process | negative regulation of viral transcription | |
Biological Process | peptidoglycan catabolic process | |
Biological Process | viral release from host cell by cytolysis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndolysin
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Kayfunavirus
Accessions
- Primary accessionA0AAE8C5I9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.
Keywords
- Cellular component
Expression
Keywords
- Developmental stage
Interaction
Subunit
Interacts with the viral RNA polymerase.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-128 | Peptidoglycan recognition protein family | ||||
Sequence: VSKVQFNPRSRTDAIFVHCSATKPEMDIGVETIRMWHKQQGWLDVGYHFIIKRDGTVEEGRPVNVVGSHVKDWNSRSVGVCLVGGIDAKGKFEANFTPAQMNSLRNKLADLKDLYPQAEIKAHHDVA | ||||||
Domain | 2-134 | N-acetylmuramoyl-L-alanine amidase | ||||
Sequence: VSKVQFNPRSRTDAIFVHCSATKPEMDIGVETIRMWHKQQGWLDVGYHFIIKRDGTVEEGRPVNVVGSHVKDWNSRSVGVCLVGGIDAKGKFEANFTPAQMNSLRNKLADLKDLYPQAEIKAHHDVAPKACPS |
Sequence similarities
Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length152
- Mass (Da)17,103
- Last updated2024-05-29 v1
- Checksum1455509DF52F75CC