A0AAE8C5C3 · A0AAE8C5C3_9CAUD

Function

function

ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination. The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis. ATP or dTTP hydrolysis propels each helicase domain to translocate sequentially along DNA. Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange. Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+, one of which is catalytic.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site18Zn2+ (UniProtKB | ChEBI)
Binding site21Zn2+ (UniProtKB | ChEBI)
Binding site37Zn2+ (UniProtKB | ChEBI)
Binding site40Zn2+ (UniProtKB | ChEBI)
Binding site158Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site208Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site239Mg2+ 2 (UniProtKB | ChEBI)
Binding site314-321ATP (UniProtKB | ChEBI)
Site362dTTP/dATP binding
Site467dTTP/dATP binding
Site506dTTP/dATP binding
Site524dTTP/dATP binding
Site537dTTP/dATP binding

GO annotations

AspectTerm
Cellular Componentprimosome complex
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA primase activity
Molecular Functionsingle-stranded DNA binding
Molecular Functionzinc ion binding
Biological Processviral DNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • ORF names
      PM131_040

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Kayfunavirus

Accessions

  • Primary accession
    A0AAE8C5C3

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homohexamer. Assembles as a hexamer onto linear or circular ssDNA in the presence of ATP or dTTP. Interacts (via C-terminus) with the viral DNA polymerase that is bound to DNA; this interaction is essential to initiate leading-strand DNA synthesis. The priming complex consists of 2 DNA polymerases and 1 helicase-primase hexamer that assemble on the DNA template. Interacts with the single-stranded DNA-binding protein. Part of the replicase complex that includes the DNA polymerase, the primase/helicase and the single-stranded DNA binding protein.

Family & Domains

Features

Showing features for zinc finger, domain.

TypeIDPosition(s)Description
Zinc finger18-40C4-like; zinc ribbon fold
Domain152-235Toprim
Domain283-550SF4 helicase

Domain

The N-terminus zinc finger domain is essential for delivering the primed DNA template to the DNA polymerase. The central core domain contains the primase activity. The C-terminus region is responsible for the helicase activity and binds 1 Mg2+-dTTP.

Sequence similarities

Belongs to the Teseptimavirus DNA helicase/primase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    566
  • Mass (Da)
    62,301
  • Last updated
    2024-05-29 v1
  • Checksum
    5A8E5FE7DB56C747
MSYDDQDDESVFLYHTQCPDCGSSDANGVYSDGHMYCFACDPSVAWKKGDMELTEGYTPSGGRKQVSNLLTFGENAGRYVPLPARSLSMEICKKYSYWVGNMGGKMVQVADYYDRSGTKVGQKVRDAEKNFTAIGSVKSDMLFGSQLWNGGKKIVITEGEIDALSVAQVQDGKYPVVSLPLGSKSAKKAMAANLEYLDQFEEIILMFDMDEPGRQAIEDAAPVLPAGRVKVAFINGYKDANAALQAKDFKAITDAIWNAKPFVPAGVVSAASLKDRTREAMLKAETEGLMFSSCTTLNAMTLGARAGELVMVTSGSGMGKSTFVRQLLLEWGRGGKRVGMAMLEEAVEETVQDLMGLDNNVRLRQSKELKQAILEDGRFDEWYDKLFGDDKFHLYDSFAESEEDTLFAKLSYMVDGLDCDVILLDHISIVVSGMEDNSDERKTIDRIMTRLKKFAKTKGVVVVVICHLKNPEKGKSHEEGRPVSITDLRGSGALRQLSDTIIALERNQQGDTPNIVQLRLLKCRFTGDTGVAGHLEYNKTTGWLEPISFTGSSGEEDSGSWEDTDF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MZ234045
EMBL· GenBank· DDBJ
QZI84391.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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