A0AAE7XKN1 · A0AAE7XKN1_9CAUD

Function

function

ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination. The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis. ATP or dTTP hydrolysis propels each helicase domain to translocate sequentially along DNA. Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange. Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+, one of which is catalytic.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site94Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site144Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site174Mg2+ 2 (UniProtKB | ChEBI)
Binding site249-256ATP (UniProtKB | ChEBI)
Site298dTTP/dATP binding
Site402dTTP/dATP binding
Site441dTTP/dATP binding
Site459dTTP/dATP binding
Site472dTTP/dATP binding

GO annotations

AspectTerm
Cellular Componentprimosome complex
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionDNA primase activity
Molecular Functionhydrolase activity
Molecular Functionmetal ion binding
Molecular Functionsingle-stranded DNA binding
Biological Processviral DNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Teetrevirus

Accessions

  • Primary accession
    A0AAE7XKN1

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homohexamer. Assembles as a hexamer onto linear or circular ssDNA in the presence of ATP or dTTP. Interacts (via C-terminus) with the viral DNA polymerase that is bound to DNA; this interaction is essential to initiate leading-strand DNA synthesis. The priming complex consists of 2 DNA polymerases and 1 helicase-primase hexamer that assemble on the DNA template. Interacts with the single-stranded DNA-binding protein. Part of the replicase complex that includes the DNA polymerase, the primase/helicase and the single-stranded DNA binding protein.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain88-175Toprim
Domain218-485SF4 helicase
Region481-504Disordered

Domain

The central core domain contains the primase activity. The C-terminus is responsible for the helicase activity.

Sequence similarities

Belongs to the Teseptimavirus DNA helicase/primase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    504
  • Mass (Da)
    55,899
  • Last updated
    2024-05-29 v1
  • Checksum
    371A0EA414AE2D9B
MSYDVWNFGDSNGRYSDLTARGISKETCQKAGYWLAKVDNRMYQVADYRDQNGSIVSQKVRDKDKNFKTTGSHKSDALFLKHLWSGGKKIVVTEGEIDALTVMELQDCKYPVVSLGHGASAAKKTCAANYEYFDQFEQIILMFDMDDAGRKAVEEAAQVLPAGKVRVAVLPYKDANECHLMGEDKAILEQVWNANPWVPDGVVSALSLKDRVKEAMTSEDAVGLLFDGCQGLNDRTLGARGGEVIMVTSGSGMGKSTFVRQQALAWGKRMGKRVGLAMLEESVEDTIQDMMGLNNKVRLRQSDEVKKAIAEDGRFDEWYDELFGDDTFHLYDSFAEAEADRLLAKLAYMRTGLGCDVIVLDHISIVVSASEESDERKMIDRLMTKLKGFAKSTGVVLVVICHLKNPEKGKPHEEGRAVSITDLRGSGALRQLSDTIIALERNQQGDMPNLVLVRLLKCRFTGDTGIAGYMEYNRETGWLEPSSYTGEEGEGDTGWTEQDGQSDF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MZ428229
EMBL· GenBank· DDBJ
QZE51104.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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