A0AAE7T056 · A0AAE7T056_ENTGA

  • Protein
    Multifunctional fusion protein
  • Gene
    ribA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 divalent metal cations per subunit. Magnesium or manganese.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.
Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site27-28D-ribulose 5-phosphate (UniProtKB | ChEBI)
Binding site28Mg2+ 1 (UniProtKB | ChEBI)
Binding site28Mg2+ 2 (UniProtKB | ChEBI)
Binding site32D-ribulose 5-phosphate (UniProtKB | ChEBI)
Site125Essential for catalytic activity
Binding site139-143D-ribulose 5-phosphate (UniProtKB | ChEBI)
Binding site142Mg2+ 2 (UniProtKB | ChEBI)
Site163Essential for catalytic activity
Binding site245-249GTP (UniProtKB | ChEBI)
Binding site250Zn2+ (UniProtKB | ChEBI); catalytic
Binding site261Zn2+ (UniProtKB | ChEBI); catalytic
Binding site263Zn2+ (UniProtKB | ChEBI); catalytic
Binding site266GTP (UniProtKB | ChEBI)
Binding site288-290GTP (UniProtKB | ChEBI)
Binding site310GTP (UniProtKB | ChEBI)
Active site322Proton acceptor
Active site324Nucleophile
Binding site345GTP (UniProtKB | ChEBI)
Binding site350GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3,4-dihydroxy-2-butanone-4-phosphate synthase activity
Molecular FunctionGTP binding
Molecular FunctionGTP cyclohydrolase II activity
Molecular Functionmagnesium ion binding
Molecular Functionmanganese ion binding
Molecular Functionzinc ion binding
Biological Processriboflavin biosynthetic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    GTP cyclohydrolase-2
  • EC number
  • Alternative names
    • GTP cyclohydrolase II
  • Recommended name
    3,4-dihydroxy-2-butanone 4-phosphate synthase
  • EC number
  • Short names
    DHBP synthase

Gene names

    • Name
      ribA
    • Synonyms
      ribB
    • ORF names
      EGM181_09990

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • EGM181
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Lactobacillales > Enterococcaceae > Enterococcus

Accessions

  • Primary accession
    A0AAE7T056

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain204-366GTP cyclohydrolase II

Sequence similarities

Belongs to the DHBP synthase family.
Belongs to the GTP cyclohydrolase II family.
In the N-terminal section; belongs to the DHBP synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    392
  • Mass (Da)
    43,800
  • Last updated
    2024-05-29 v1
  • Checksum
    A45F06D9238785C0
MSKNKIETALDYLRNGQLIIVADDDDREAEGDLVGIANLATMESVNFMTKFARGLICAPISAQLADRLQLTEMVAQNTDTFKTAFTISVDHRSTTTGISAEERARTIRALANPQTKEEDFQKPGHIFPLIGRIGGVLERRGHTEAALDLAKLTNQSEAAYICEILNEDGTMARRPQLAQLAEKWQLPMITIEELATYMATNQLTVQLPTAFGSFDLTLFEDQHHLEHLVLAKGDLTNPAKPLLVRLHSECLTGDVFGSQRCDCGEQLHEAMRQIEKEGTGAILYLRQEGRGIGLKQKLQAYQLQEKGLDTYEANVELGFRPDERDYGFAAIILKSLGIDTIRLLTNNPEKITELEAHGIHVAERIPLEITPVADNNRYLQTKKEKFHHLLSL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP050485
EMBL· GenBank· DDBJ
QOG27560.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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