A0AAE3FJ10 · A0AAE3FJ10_9CREN
- ProteinBifunctional NAD(P)H-hydrate repair enzyme
- GenennrD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids501 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.
Catalytic activity
- (6R)-NADHX = (6S)-NADHX
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 123 | K+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 127-133 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: GTGFSGK | ||||||
Binding site | 156 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 159 | K+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 246 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 314 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 363 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 428 | AMP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 429 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ADP-dependent NAD(P)H-hydrate dehydratase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | NADHX epimerase activity | |
Biological Process | metabolite repair | |
Biological Process | nicotinamide nucleotide metabolic process |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameBifunctional NAD(P)H-hydrate repair enzyme
- Alternative names
Including 2 domains:
- Recommended nameADP-dependent (S)-NAD(P)H-hydrate dehydratase
- EC number
- Alternative names
- Recommended nameNAD(P)H-hydrate epimerase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Candidatus Aramenus
Accessions
- Primary accessionA0AAE3FJ10
Proteomes
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 7-208 | YjeF N-terminal | ||||
Sequence: MRALEVNSEALGVPTLLLMENAGRSVKDEVVRALGDVKGKTVYAFVGHGGKGGDALVAVRHLVGEGARAVVLLLGENKHKDAVFNLSVIREMDYSVKLVEVKDVEQLSPVEGDVLIDGMLGTGFSGKPREPFRTAINVFNRSKGFKVSIDVPSGIDSDTGEAPGEFVVPDLVVTFHDLKPGLAKRGFNVVVKGIGIPPEAYI | ||||||
Domain | 211-487 | YjeF C-terminal | ||||
Sequence: GPGDLLTRVKKRDMKSKKGAGGRVLIIGGSKTYSGAPSLSALASLKTGADLVYVASPENTANIVASYSPDLIAIKLKGENINPKNLEELKPWIEKVNVVVIGPGMGLEEETVEASKAIVEYLKEKGKKAVVDADALKAIKGARLTKDFVITPHAGEFKIFFGEEPSQDPRERITQAEARARDCNCVVLLKGYFDVITDGERTKLNKTGNPGMTVGGTGDTLTGIVATLMAQGIEPFYAAAIGAFVNGLAGSLVYSTVGNHFTPTELVAEIPKVLQNP |
Sequence similarities
Belongs to the NnrD/CARKD family.
Belongs to the NnrE/AIBP family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length501
- Mass (Da)53,400
- Last updated2024-05-29 v1
- Checksum31C73862564C54C6