A0AAE1HDE5 · A0AAE1HDE5_9NEOP
- ProteinFlap endonuclease 1
- GeneFen1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1829 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Catalytic activity
- xanthine + NAD+ + H2O = urate + NADH + H+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleolus | |
Cellular Component | nucleoplasm | |
Cellular Component | peroxisome | |
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | 5'-3' exonuclease activity | |
Molecular Function | 5'-flap endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | FAD binding | |
Molecular Function | iron ion binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | molybdenum ion binding | |
Molecular Function | xanthine dehydrogenase activity | |
Biological Process | base-excision repair | |
Biological Process | DNA replication, removal of RNA primer |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFlap endonuclease 1
- EC number
- Short namesFEN-1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Paraneoptera > Thysanoptera > Terebrantia > Thripoidea > Thripidae > Frankliniella
Accessions
- Primary accessionA0AAE1HDE5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for coiled coil, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 122-149 | |||||
Sequence: LNKRAERREEAQKALQKAEEAGDAENID | ||||||
Region | 374-401 | Disordered | ||||
Sequence: SPSGGQKRKSEAAKNTPNKRGKKGGSFR | ||||||
Domain | 420-507 | 2Fe-2S ferredoxin-type | ||||
Sequence: STLVFFVNGKKVVDDAVDPEWTLLYYLRNKLRLCGTKLGCAEGGCGACTVMVSKLDRTTKKIAHLAVNACLAPVCAMHGLAVTTVEGI | ||||||
Region | 588-630 | Disordered | ||||
Sequence: STVRTHSLSNGKANGGVNGQSNGQSNGQSNGHSNGVNGHSNGV | ||||||
Domain | 709-895 | FAD-binding PCMH-type | ||||
Sequence: IKGPRVTWHRPTSLDTLLELKAKHSDAKIIVGNTEVGVETKFKNCLYPVEIMPSRIPELTAIERLPDAVRVGASVTLRDMEDYFLGVMKEEPESRTRIMKAIVAMLHYFAGRQIRNVGAIGGNVMTGSPISDMLPILLASNVNMEVRSKERGSRQIKLDHTFFTGYRRNVVKPDEILVSLDVPFTHA |
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Belongs to the xanthine dehydrogenase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,829
- Mass (Da)201,391
- Last updated2024-05-29 v1
- ChecksumD83BFE923AB711E7
Keywords
- Technical term