A0AAD9GK15 · A0AAD9GK15_BABDI
- ProteinProbable ATP-dependent 6-phosphofructokinase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1342 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H+
Cofactor
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 204 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 268-269 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RG | ||||||
Binding site | 297-300 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GDGS | ||||||
Binding site | 298 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Site | 299 | Important for substrate specificity; cannot use PPi as phosphoryl donor | ||||
Sequence: G | ||||||
Binding site | 338-340 | substrate | ||||
Sequence: TVD | ||||||
Active site | 340 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 385-387 | substrate | ||||
Sequence: MGR | ||||||
Binding site | 446 | substrate | ||||
Sequence: E | ||||||
Binding site | 548-551 | substrate | ||||
Sequence: YEGR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | diphosphate-fructose-6-phosphate 1-phosphotransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable ATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Piroplasmida > Babesiidae > Babesia
Accessions
- Primary accessionA0AAD9GK15
Proteomes
Subcellular Location
Interaction
Subunit
Tetramer of two alpha (regulatory) and two beta (catalytic) chains.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 48-72 | Disordered | ||||
Sequence: RRDRFRISSRSTSSHQSESRHRSVS | ||||||
Compositional bias | 57-71 | Polar residues | ||||
Sequence: RSTSSHQSESRHRSV | ||||||
Domain | 196-450 | Phosphofructokinase | ||||
Sequence: KIGLVLSGGPAPGGHNVIAGIFDYMYLRNTQSQMIGFHGGLDGLLNRHHEIITPHAMDQFRNMGGFNMLLSGRGKISGASDLDKAVEAVNELDLDGLIIVGGDGSNSNAAILANHFAGRANVTNPDGKPALKKPCCVVGIPKTVDGDVKSHNIEISFGFDTAARTYSELIGNLCTDASSTQYTYHFIRVMGRSASHLALECGMQTHPNVVLIGEEVKRKQQSLSAIVDDIVDLMEKRHSMGKPYGVVLIPEGLIE | ||||||
Domain | 776-1014 | Phosphofructokinase | ||||
Sequence: KVGVVVLSKQAPGVLNVIWGLHERLSIYGSKCFAFNGGKGLVDCDYIELCANDFDLFRNQGGLEMVHRSRVHYFHDPKHWGPALQTCTKLGLDGLVILGDEVAMTQAALLTEYFLSKNSQICIVGVPVAGSNGLACQLIESCVGFDSNAQLYASLVGNVLTDAVSMPKYWHFVKILGRYPSLEVLECALQTHPNFIIIAEEYGSADKTLFDVVRDIADAVCKRAKAGKNFGTVLIPDHL |
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'Long' sub-subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,342
- Mass (Da)149,449
- Last updated2024-05-29 v1
- Checksum6569AEA6156CE710
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 57-71 | Polar residues | ||||
Sequence: RSTSSHQSESRHRSV |
Keywords
- Technical term