A0AAD8BHE5 · A0AAD8BHE5_BIOPF
- ProteinLanosterol 14-alpha demethylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids494 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
Catalytic activity
- 24,25-dihydrolanosterol + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 4 H+ + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
CHEBI:28113 + 3 CHEBI:15379 + 3 RHEA-COMP:11964 = CHEBI:78904 + CHEBI:15740 + 4 CHEBI:15378 + 4 CHEBI:15377 + 3 RHEA-COMP:11965 - 24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-hydroxy-24,25-dihydrolanosterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-hydroxy-24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-oxo-24,25-dihydrolanosterol + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-hydroxylanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-oxolanosterol + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-oxo-24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-oxolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- a 14alpha-formyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a Delta14 steroid + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- a 14alpha-hydroxymethyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a 14alpha-formyl steroid + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- a 14alpha-methyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a 14alpha-hydroxymethyl steroid + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- lanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-hydroxylanosterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Pathway
Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | steroid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLanosterol 14-alpha demethylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Spiralia > Lophotrochozoa > Mollusca > Gastropoda > Heterobranchia > Euthyneura > Panpulmonata > Hygrophila > Lymnaeoidea > Planorbidae > Biomphalaria
Accessions
- Primary accessionA0AAD8BHE5
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length494
- Mass (Da)56,297
- Last updated2024-05-29 v1
- Checksum588B9348AA8CC71F
Keywords
- Technical term