A0AAD5VZ36 · A0AAD5VZ36_9AGAR
- ProteintRNA (guanine(37)-N1)-methyltransferase
- GeneTRM5
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1155 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Catalytic activity
- guanosine37 in tRNA + S-adenosyl-L-methionine = H+ + N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 225 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 263-264 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DL | ||||||
Binding site | 293-294 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DA | ||||||
Binding site | 314 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Cellular Component | mitochondrial matrix | |
Cellular Component | nucleus | |
Molecular Function | calcium ion binding | |
Molecular Function | L-aspartate transmembrane transporter activity | |
Molecular Function | L-glutamate transmembrane transporter activity | |
Molecular Function | tRNA (guanine(37)-N1)-methyltransferase activity | |
Biological Process | malate-aspartate shuttle | |
Biological Process | tRNA methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine(37)-N1)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Agaricomycetidae > Agaricales > Agaricineae > Agaricaceae > Leucocoprinus
Accessions
- Primary accessionA0AAD5VZ36
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Mitochondrion inner membrane ; Multi-pass membrane protein
Note: Predominantly in the mitochondria and in the nucleus.
Keywords
- Cellular component
Interaction
Subunit
Homodimer (via N-terminus).
Monomer.
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-21 | Disordered | ||||
Sequence: MSTHAYLDASPPPYNGPTNRL | ||||||
Domain | 136-405 | SAM-dependent methyltransferase TRM5/TYW2-type | ||||
Sequence: FAMTGHIAHVNLLDEYLPYKYLIGQLILDKNKKVRTVVNKLNSIDTQFRFFKMEVIAGEQDYIVEHHESDCRFTFDFSKVYWNSRLHTEHERLVQSFGPEEVVADVFAGVGPFAIPAARKGCAVLANDLNPASHEYLEKNVHDNRDRENRRARALQKDANNLTKSDLPSRKRIGHFVMNLPDSAISFLDAFRGILAGEESHGLASTYEVTPMVHCHCFTRELDPQKAAADIRQRVEQQLGCPVDEDVSLHLVRSVAPNKEMYCISFRLPR | ||||||
Compositional bias | 275-292 | Basic and acidic residues | ||||
Sequence: NVHDNRDRENRRARALQK | ||||||
Region | 275-301 | Disordered | ||||
Sequence: NVHDNRDRENRRARALQKDANNLTKSD | ||||||
Domain | 569-604 | EF-hand | ||||
Sequence: RPDADYWIAFQFFDVDHSGNISFEEFKSVFSANIGP | ||||||
Domain | 641-676 | EF-hand | ||||
Sequence: LQGERVRQAFKYLDKDQDGYISPEQFKRIILEIAGH | ||||||
Repeat | 807-897 | Solcar | ||||
Sequence: LQSAYSFIQGGIAGAFGATIVYPIDMVKTRMQNQRSTVVGQLLYKNSIDCARKILRNEGFLGFYRGLGPQLVGVAPEKAIKLTVNDLIRSR | ||||||
Repeat | 906-993 | Solcar | ||||
Sequence: IKVFWELVAGGMAGGCQVVFTNPLEIVKIRLQVQGEAAKVEGIKPRGAVHIIRQLGILGLYRGASACLLRDIPFSAIYFPAYSHLKTD | ||||||
Repeat | 1004-1092 | Solcar | ||||
Sequence: LSFTETLAAAAIAGMPAAYLTTPADVVKTRLQVEARQGQTHYKGLRDAFVKIYREEGFRALFKGGPARVIRSSPQFGFTLLGYETLKTL |
Sequence similarities
Belongs to the TRM5 / TYW2 family.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Belongs to the mitochondrial carrier (TC 2.A.29) family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,155
- Mass (Da)129,579
- Last updated2024-05-29 v1
- Checksum621DFD091F5CB988
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 275-292 | Basic and acidic residues | ||||
Sequence: NVHDNRDRENRRARALQK |
Keywords
- Technical term