A0AAD5VZ36 · A0AAD5VZ36_9AGAR

  • Protein
    tRNA (guanine(37)-N1)-methyltransferase
  • Gene
    TRM5
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site225S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site263-264S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site293-294S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site314S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial inner membrane
Cellular Componentmitochondrial matrix
Cellular Componentnucleus
Molecular Functioncalcium ion binding
Molecular FunctionL-aspartate transmembrane transporter activity
Molecular FunctionL-glutamate transmembrane transporter activity
Molecular FunctiontRNA (guanine(37)-N1)-methyltransferase activity
Biological Processmalate-aspartate shuttle
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA (guanine(37)-N1)-methyltransferase
  • EC number
  • Alternative names
    • M1G-methyltransferase
    • tRNA [GM37] methyltransferase
    • tRNA methyltransferase 5

Gene names

    • Name
      TRM5
    • ORF names
      NP233_g2362

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • VT141
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Agaricomycetidae > Agaricales > Agaricineae > Agaricaceae > Leucocoprinus

Accessions

  • Primary accession
    A0AAD5VZ36

Proteomes

Subcellular Location

Membrane
; Multi-pass membrane protein
Mitochondrion inner membrane
; Multi-pass membrane protein
Mitochondrion matrix
Nucleus
Cytoplasm
Note: Predominantly in the mitochondria and in the nucleus.

Keywords

Interaction

Subunit

Homodimer (via N-terminus).
Monomer.

Family & Domains

Features

Showing features for region, domain, compositional bias, repeat.

TypeIDPosition(s)Description
Region1-21Disordered
Domain136-405SAM-dependent methyltransferase TRM5/TYW2-type
Compositional bias275-292Basic and acidic residues
Region275-301Disordered
Domain569-604EF-hand
Domain641-676EF-hand
Repeat807-897Solcar
Repeat906-993Solcar
Repeat1004-1092Solcar

Sequence similarities

Belongs to the TRM5 / TYW2 family.
Belongs to the mitochondrial carrier (TC 2.A.29) family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,155
  • Mass (Da)
    129,579
  • Last updated
    2024-05-29 v1
  • Checksum
    621DFD091F5CB988
MSTHAYLDASPPPYNGPTNRLDKSVFRKTLTVLAARVPPSKTGQLLKAQSLKGTLIDLPKVRSVSSNSENPEGDRLVLLRMSRIADIPREAKEYLDKETHGLVDFKIDLDYDHWTADDIMQQVLPPELREGAPTGFAMTGHIAHVNLLDEYLPYKYLIGQLILDKNKKVRTVVNKLNSIDTQFRFFKMEVIAGEQDYIVEHHESDCRFTFDFSKVYWNSRLHTEHERLVQSFGPEEVVADVFAGVGPFAIPAARKGCAVLANDLNPASHEYLEKNVHDNRDRENRRARALQKDANNLTKSDLPSRKRIGHFVMNLPDSAISFLDAFRGILAGEESHGLASTYEVTPMVHCHCFTRELDPQKAAADIRQRVEQQLGCPVDEDVSLHLVRSVAPNKEMYCISFRLPRAYKDNEQKPSITSDDYDRAGSSRRSVKGNQSWLAPLSVYRPLSGLFPHFTYPVYIMASSSPIVPTIHADADSKLSSALRSVRTAVAVPESELKRWRRTFEANAKVVNGEKFLDPESFVNAIAPKGDLTKIDRAQFSVLFRVADTSRRGLVSWEDFTVFETLLKRPDADYWIAFQFFDVDHSGNISFEEFKSVFSANIGPDSIPFDFDCDWVKLYLGKKDGTHVLGYNEFTQLMKGLQGERVRQAFKYLDKDQDGYISPEQFKRIILEIAGHKLSDAVIDRLPTLCTLTPGQRINYSEVIAFHNVLREMDMVERIIREATAKSKDGRIDQTDFLNHSSSNSRYSLFTPMEASIIFHFAGRGIATQRLTLLDFAQLLDPRWKAPHSELETRSPGTATSILNTFLQSAYSFIQGGIAGAFGATIVYPIDMVKTRMQNQRSTVVGQLLYKNSIDCARKILRNEGFLGFYRGLGPQLVGVAPEKAIKLTVNDLIRSRAMDPETGRIKVFWELVAGGMAGGCQVVFTNPLEIVKIRLQVQGEAAKVEGIKPRGAVHIIRQLGILGLYRGASACLLRDIPFSAIYFPAYSHLKTDLFHEGYNGKKLSFTETLAAAAIAGMPAAYLTTPADVVKTRLQVEARQGQTHYKGLRDAFVKIYREEGFRALFKGGPARVIRSSPQFGFTLLGYETLKTLFPYPWQDAPPQVNTALTSRADELAQVRARNALKILLDVHGDFGRRATTSGTPLSLTHPRLSPP

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias275-292Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JANIEX010000099
EMBL· GenBank· DDBJ
KAJ3573542.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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