A0AAD5L8I0 · A0AAD5L8I0_PYTIN
- ProteinDNA repair nuclease/redox regulator APEX1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids335 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Probably binds two magnesium or manganese ions per subunit.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 110 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 186 | |||||
Sequence: Y | ||||||
Active site | 226 | Proton donor/acceptor | ||||
Sequence: D | ||||||
Binding site | 226 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 228 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Site | 228 | Transition state stabilizer | ||||
Sequence: N | ||||||
Site | 298 | Important for catalytic activity | ||||
Sequence: D | ||||||
Binding site | 323 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 324 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 324 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 324 | Interaction with DNA substrate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | double-stranded DNA 3'-5' DNA exonuclease activity | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoric diester hydrolase activity | |
Biological Process | base-excision repair |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA repair nuclease/redox regulator APEX1
- EC number
- Short namesAPEN ; REF-1
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Oomycota > Pythiales > Pythiaceae > Pythium
Accessions
- Primary accessionA0AAD5L8I0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-41 | Disordered | ||||
Sequence: MPPPPTTTTRSRRRKKRKRPPVTTKKGKKKNAGDGNWSRPA | ||||||
Compositional bias | 9-26 | Basic residues | ||||
Sequence: TRSRRRKKRKRPPVTTKK | ||||||
Domain | 76-324 | Endonuclease/exonuclease/phosphatase | ||||
Sequence: AWNVNGLRALLKKDDSIHFRAYAAQEDADVLCLSEIKIDRNEVEKLEDVLPQYEHQFWSSAAKKGYSGTAIFSKIKPLSVKDEIIVEGDAPEDEGRFLALEFATFWLVHTYVPNAGQKLDRLSYRTEKWDKAMLRMLKDLEASKPVVWCGDLNVAHQEIDIHDPKGNRNKSPGFTDAERDSFGEILASGFVDTFRHLHPEQQQYTYWSYRFNARAKNKGWRLDYFVVSQSLLARVHQSFVRDAITGSDH |
Sequence similarities
Belongs to the DNA repair enzymes AP/ExoA family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length335
- Mass (Da)38,086
- Last updated2024-05-29 v1
- Checksum4DF63390C49F685A
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 9-26 | Basic residues | ||||
Sequence: TRSRRRKKRKRPPVTTKK |
Keywords
- Technical term