A0AAD4QYV0 · A0AAD4QYV0_9BILA
- ProteinAcireductone dioxygenase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids182 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
Catalytic activity
- 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H+
Cofactor
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )
Note: Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 88 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 88 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 90 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 90 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 94 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: E | ||||||
Binding site | 94 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: E | ||||||
Binding site | 133 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 133 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | acireductone dioxygenase (Ni2+-requiring) activity | |
Molecular Function | acireductone dioxygenase [iron(II)-requiring] activity | |
Molecular Function | iron ion binding | |
Molecular Function | nickel cation binding | |
Biological Process | L-methionine salvage from methylthioadenosine |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameAcireductone dioxygenase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Tylenchina > Tylenchomorpha > Sphaerularioidea > Anguinidae > Anguininae > Ditylenchus
Accessions
- Primary accessionA0AAD4QYV0
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length182
- Mass (Da)21,589
- Last updated2024-05-29 v1
- Checksum8AF38B158F17D36C
Keywords
- Technical term