A0AAD4LQI7 · A0AAD4LQI7_9AGAM
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1193 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- ATP + hydrogencarbonate + pyruvate = ADP + H+ + oxaloacetate + phosphate
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 156 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 240 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 275 | ATP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 332 | |||||
Sequence: R | ||||||
Binding site | 588 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 660 | substrate | ||||
Sequence: R | ||||||
Binding site | 757 | Mn2+ (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 787 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 789 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 922 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Russulales > Russulaceae > Lactarius
Accessions
- Primary accessionA0AAD4LQI7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 757 | N6-carboxylysine | ||||
Sequence: K | ||||||
Modified residue | 1158 | N6-biotinyllysine | ||||
Sequence: K |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 40-490 | Biotin carboxylation | ||||
Sequence: PLTKILVANRGEIAVRVFRTAHELAMHTVAIYSYEDRMSAHRQKADEAYRVGAGLTPVAAYLAQDDIIRIALEHGVDMIHPGYGFLSENAEFARKVEQAGIAFVGPSPEVIDGLGDKTKARSLAIKVGVPVVPGTPGAVDKWSDADSFIKEYGFPVIIKAAMGGGGRGMRVVREESEFKNAFERAVSEAKSAFGDGTVFIERFLERPRHIEVQILGDSQGNIVHLFERDCSVQRRHQKVVEVAPATHLPEEVRQAIWADAIKLAKSVNYRNAGTAEFLVDQQGRHYFIEINPRIQVEHTITEEITGLDIVAAQIQIAAGATLPQLGLTQDAITKRGFAIQCRVTTEDAAANFQPDTGKIEVYRSAGGNGVRLDASSGFAGAQITPHYDSLLVKVSVSGTTYEVARRKMLRALVEFRIRGVKTNIPFLFRLLTHDVFIGGKTWTTFIDDTPE | ||||||
Domain | 160-357 | ATP-grasp | ||||
Sequence: RSLAIKVGVPVVPGTPGAVDKWSDADSFIKEYGFPVIIKAAMGGGGRGMRVVREESEFKNAFERAVSEAKSAFGDGTVFIERFLERPRHIEVQILGDSQGNIVHLFERDCSVQRRHQKVVEVAPATHLPEEVRQAIWADAIKLAKSVNYRNAGTAEFLVDQQGRHYFIEINPRIQVEHTITEEITGLDIVAAQIQIAA | ||||||
Domain | 579-848 | Pyruvate carboxyltransferase | ||||
Sequence: TLIMDTTWRDAHQSLLATRLRTIDIANIAKETSWALSNAYSLECWGGATFDVAMRFLYEDPWERLRAIRRLVPNIPLQALVRGANGVGYTSYPDNAIYDFSKKAVENGLDIFRVFDSLNYIENMKLGIDAAKKAGGVVEAVVCYSGDVASKTETKYTLDYYLNFVEQIVAEGIHVLGIKDMAGLLKPESAKLLVGSIRAKYPDLPIHVHSHDTAGISTASMLAAAAAGADVVDVAIDSMSGLTSQPPMGAISLALEQTGLGTGIRYADIQ | ||||||
Domain | 1114-1192 | Lipoyl-binding | ||||
Sequence: VSREKATSDPGSVGAPMSGVVVEVRVKEGQEIKKGDPLCILSAMKMESAVTAPVSGHIKRVVVHEGDSINQGDLTVEIV |
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,193
- Mass (Da)130,365
- Last updated2024-05-29 v1
- ChecksumFCB2A784BAD37472
Keywords
- Technical term