A0AAD1NP79 · A0AAD1NP79_9GAMM
- ProteinPhosphoenolpyruvate-protein phosphotransferase
- GeneptsI
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids567 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
Catalytic activity
- L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 188 | Tele-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Binding site | 295 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 331 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 429 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 452-453 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: ND | ||||||
Binding site | 453 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 463 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 500 | Proton donor | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoenolpyruvate-protein phosphotransferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate-protein phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Alteromonadales > Shewanellaceae > Shewanella
Accessions
- Primary accessionA0AAD1NP79
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-125 | Phosphotransferase system enzyme I N-terminal | ||||
Sequence: GIAVSSGIAFGEAVVYNPFDNSMDYRLLPLAKIPQELNKLTQALQRLCQQLQTSLDKLTEDSDNFQLVEADLLLLEDAELIQQIRESISGLQFSATAAVERVFAHQASELEALEDPYLANR | ||||||
Domain | 152-224 | PEP-utilising enzyme mobile | ||||
Sequence: KHDSILLAQDLTPAEFALLPLEHIKGIILKTGGLTSHTAILARAAGIPALLSCNYQTGNINAGDPLILDANQG | ||||||
Domain | 252-536 | PEP-utilising enzyme C-terminal | ||||
Sequence: LEQYRDRQTQTQDGKRISLMANVGNLNEISRLPQVGAEGIGLFRTEFMLTDVSCIPDEKAQYNLYCDALQLLNGQPFTIRTLDIGADKELPCLDQAIEDNPALGMRGVRYTLKHPEILQTQLRAVLRAANHGPVRLMLPMVNQVEELDAVLDMLEHCKQQLSEAEKGYGEITLGIVVETPAAVMNLPTLLPKLDFISIGTNDLTQYAMAADRTNPELTRDYPSFSPAILRLIAMTIADAKAADIRVSLCGELAGDPRIAPILMGMGLDELSVNPGSVLEVKAALC |
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length567
- Mass (Da)62,065
- Last updated2024-05-29 v1
- ChecksumBD81E97DA4A43BA0