A0AAD1B4E9 · A0AAD1B4E9_9PSED

  • Protein
    Adenylosuccinate synthetase
  • Gene
    purA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site13-19GTP (UniProtKB | ChEBI)
Active site14Proton acceptor
Binding site14Mg2+ (UniProtKB | ChEBI)
Binding site14-17IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site39-42IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site41Mg2+ (UniProtKB | ChEBI)
Binding site41-43GTP (UniProtKB | ChEBI)
Active site42Proton donor
Binding site130IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Active site141
Binding site144IMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site225IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site240IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site300-306substrate
Binding site304IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site306GTP (UniProtKB | ChEBI)
Binding site332-334GTP (UniProtKB | ChEBI)
Binding site414-416GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionadenylosuccinate synthase activity
Molecular FunctionGTP binding
Molecular Functionmagnesium ion binding
Biological Processpurine nucleotide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Adenylosuccinate synthetase
  • EC number
  • Short names
    AMPSase
    ; AdSS
  • Alternative names
    • IMP--aspartate ligase

Gene names

    • Name
      purA
    • ORF names
      PST29_0573

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • St29
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0AAD1B4E9

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the adenylosuccinate synthetase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    430
  • Mass (Da)
    46,820
  • Last updated
    2024-05-29 v1
  • Checksum
    EA76C7FCD045B5C6
MGKNVVVLGTQWGDEGKGKIVDLLTEHAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGVLREGVQCLIGNGVVVAPDALMREIVKLEEKGVPVRERLRISPSCPLILSYHVALDQAREKARGEQKIGTTGRGIGPAYEDKVARRGLRIGDLFHRERFAAKLGELLDYHNFVLVNYYKEPAIDFQKTLDECMEYAELLKPMMLDVTAELHQLRRAGKDIMFEGAQGSLLDIDHGTYPYVTSSNTTAGGIATGSGVGPMYLDYILGITKAYTTRVGSGPFPTELFDDVGAFLAKRGHEFGATTGRARRCGWFDAVILRRAIDVNSISGLCLTKLDVLDGLETINICVGYKNQDGAVIDAPTDADSYIGLEPVYEQMPGWSESTLGAKTLEELPAAAQAYIKRIEELVGAPIDIISTGPDRNETIVLRHPFA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP014628
EMBL· GenBank· DDBJ
BAQ78462.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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