A0AAD0IAB0 · A0AAD0IAB0_PSESX

Function

function

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site233substrate
Binding site262substrate
Binding site291substrate
Binding site327substrate
Binding site347-349substrate
Binding site388-391substrate
Binding site427substrate
Binding site431Zn2+ (UniProtKB | ChEBI)
Binding site454substrate
Binding site495Zn2+ (UniProtKB | ChEBI)
Binding site575[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site578[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site583[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-carbon lyase activity
Molecular Functionzinc ion binding
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphomethylpyrimidine synthase
  • EC number
  • Alternative names
    • Hydroxymethylpyrimidine phosphate synthase
      (HMP-P synthase
      ; HMP-phosphate synthase
      ; HMPP synthase
      )
    • Thiamine biosynthesis protein ThiC

Gene names

    • Name
      thiC
    • ORF names
      DA456_16725

Organism names

  • Taxonomic identifier
  • Strain
    • LMG5095
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas > Pseudomonas syringae

Accessions

  • Primary accession
    A0AAD0IAB0

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-23Disordered
Compositional bias7-23Polar residues
Domain24-101ThiC-associated

Sequence similarities

Belongs to the ThiC family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    629
  • Mass (Da)
    69,590
  • Last updated
    2024-05-29 v1
  • Checksum
    B2816EE65794AC50
MSIKAKNAAHLSESAQVDSGSVQPFTRSQKIYVQGSRPDIRVPMREITLDVTPTDFGGEINAPVTIYDTSGPYTDPNVIIDVRKGLADVRSPWIDSRNDTERLPGLSSNFGQQRLSDAELTALRFAHVRNPRRAKAGANVSQMHYARQGIITAEMEYVAIRENMKLQEARAAGLLTQQHAGHSFGASIPKEITAEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGAAEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKHDVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIEALDVDVAKGLAEQAERFKQEGSQLYKKV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias7-23Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP028490
EMBL· GenBank· DDBJ
AVX24911.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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