A0AAC9AN68 · A0AAC9AN68_9ACTN

Function

function

Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site990Mg2+ 1 (UniProtKB | ChEBI)
Binding site997Mg2+ 2 (UniProtKB | ChEBI)
Binding site997Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionlysine-tRNA ligase activity
Molecular Functionmagnesium ion binding
Molecular Functiontransferase activity
Molecular FunctiontRNA binding
Biological Processlipid metabolic process
Biological Processlysyl-tRNA aminoacylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lysine--tRNA ligase
  • EC number
  • Alternative names
    • Lysyl-tRNA synthetase
      (LysRS
      )

Gene names

    • Name
      lysS
    • ORF names
      AXH35_04755

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 55737
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Propionibacteriales > Propionibacteriaceae > Acidipropionibacterium

Accessions

  • Primary accession
    A0AAC9AN68

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane25-48Helical
Transmembrane60-82Helical
Transmembrane102-123Helical
Transmembrane135-156Helical

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain755-1077Aminoacyl-transfer RNA synthetases class-II family profile

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,081
  • Mass (Da)
    117,795
  • Last updated
    2024-05-29 v1
  • Checksum
    41737D41C53FDE11
MLVGAVLLIGRMLFQQTWWVSALATGFSIVVFPIDGGGWGLVVLLLILAAGLNRRKRLSWAVCLGLFGLMWVVSLLFFGVLIAEVAGGEPTRIHDDVEMAGYVFNTFSVGAILVALIVHHGRFGARTARANVRPALATLVGGLAATVLIGWLLVWATGGQGRPRNRLWRLISELLRGNRSQVAPPSPIWVENIVELLAAITLATAFIMLLRSQRQVAVLGLGDELELRNLLDENPSDSLGYFALRRDKAVVFSPDRRAAVCYRAVAGVALASGDPVGPRAQWPAAIDAFLTVAHTYGWSPAVVGASEEGATAWQGAGLRVTRIGDEAIISPSTFDLQSRDLRGVRSVVNRLRRSGYTVRVRRHSDIEPDELSRLIALADAWRADGEERGFSMALSRLGDPLDGDCVMVEALYPAGDIRGDGSTAGLLSFVPWGGDGLSLDVMRHDPRADNGVTELMVAGLMATGRELGLHRVSLNFAVFREVIEEGSRVGARPLQRLAARFVSGVSRWFQIEQLYRSNVKYAPDWQARYLGYADAGELAQVGLALGLAEGQIDLPRILRPAPPPAQPIYSVAAHPEIAAVLERQRPDVELPGRRVGEQMRHRQQTRERIIAAGGEAYPPDIHPSGAPGDLATAAEGGELSLVGRVAGVRDHGGVIFADLTDWSGTAQILLDASALGAPAMDRWRADISLGDHVLVGGRAGSSRNGTRSLLAGEFRLAAKALHPLPDRRSGMSDPEEKVRRRYLDLIVNPAARDELRARSQAIRAVRETLLSHDFLEVETPILQTIHGGANARPFRTHINAYDLDLYLRIAPELYLKRLMVGGAGRVFEIGRNFRNEGADATHNPEFTMLEAYQAYGDYVQMRHLTREMILAASRRATGGTVIHGRDADGVEHEMDLAEEWRVITVNEGISAALGEEVTADTPKETLVGYAEKLGIAVSPKWDRGDVVLELHEHLSERTTVGPTFFCDFPADVSPLTRQHRDDPRLAEKWDLIVLGDEVATAYTELVDPVIQRERFTAQSLRAAGGDPEAMELDEDFLEALEYAMPPTGGMGMGLDRLVMLLTGASIRQTITFPLVRPRSSS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP014352
EMBL· GenBank· DDBJ
AMS04886.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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