A0AAA9TFA4 · A0AAA9TFA4_BOVIN

  • Protein
    Polyunsaturated fatty acid lipoxygenase ALOX15
  • Gene
    ALOX15
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = (8S)-hydroxy-(11S,12S)-epoxy-(5Z,9E,14Z)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(14R,15S)-epoxy-(5Z,8Z,10E)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (5S)-hydroperoxy-(14R,15S)-epoxy-(6E,8Z,11Z)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14S,15R)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(14S,15R)-epoxy-(5Z,8Z,10E)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (14S,15R)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (8S)-hydroperoxy-(14S,15R)-epoxy-(5Z,9E,11Z)-eicosatrienoate
    This reaction proceeds in the forward direction.
  • (15R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2O
    This reaction proceeds in the forward direction.
  • (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = (14S,15S)-epoxy-(5Z,8Z,10E,12E)-eicosatetraenoate + H2O
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate + O2 = 14-hydroperoxy-(4Z,7Z,10Z,12E,16Z)-docosapentaenoate
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (11S)-hydroperoxy-(4Z,7Z,9E,13Z,16Z,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (14S)-hydroperoxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (17S)-hydroperoxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = (14R,15S)-dihydroperoxy-(5Z,8Z,10E,12E)-eicosatetraenoate
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = (8S,15S)-dihydroperoxy-(5Z,9E,11Z,13E)-eicosatetraenoate
    This reaction proceeds in the forward direction.
  • (7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S,14S)-dihydroperoxy-(4Z,8E,10Z,12E,16Z,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S,17S)-dihydroperoxy-(4Z,8E,10Z,13Z,15E,19Z)-docosahexaenoate
    This reaction proceeds in the forward direction.
  • (7Z,10Z,13Z,16Z,19Z)-docosapentaenoate + O2 = 14-hydroperoxy-(7Z,10Z,12E,16Z,19Z)-docosapentaenoate
    This reaction proceeds in the forward direction.
  • (9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate
    This reaction proceeds in the forward direction.
    EC:1.13.11.12 (UniProtKB | ENZYME | Rhea)
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-alanine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-alanine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-gamma-aminobutanoate
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-gamma-aminobutanoate
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-glycine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-glycine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-taurine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-taurine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-taurine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-taurine
    This reaction proceeds in the forward direction.

Cofactor

Fe cation (UniProtKB | Rhea| CHEBI:24875 )

Note: Binds 1 Fe cation per subunit.

Pathway

Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site54Ca2+ 1 (UniProtKB | ChEBI)
Binding site55Ca2+ 1 (UniProtKB | ChEBI)
Site80Essential for stabilizing binding to COTL1
Binding site341Fe cation (UniProtKB | ChEBI); catalytic
Binding site346Fe cation (UniProtKB | ChEBI); catalytic
Binding site521Fe cation (UniProtKB | ChEBI); catalytic
Binding site643Fe cation (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentlipid droplet
Cellular Componentplasma membrane
Molecular Functionarachidonate 12(S)-lipoxygenase activity
Molecular Functioniron ion binding
Molecular Functionlipid binding
Biological Processarachidonic acid metabolic process
Biological Processlipid oxidation
Biological Processlipoxygenase pathway

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Polyunsaturated fatty acid lipoxygenase ALOX15
  • EC number
  • Alternative names
    • 12/15-lipoxygenase
    • Arachidonate 12-lipoxygenase, leukocyte-type
    • Arachidonate 15-lipoxygenase
    • Arachidonate omega-6 lipoxygenase
    • Hepoxilin A3 synthase Alox15
    • Linoleate 13S-lipoxygenase

Gene names

    • Name
      ALOX15

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Hereford
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    A0AAA9TFA4

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Peripheral membrane protein
Cytoplasm, cytosol
Lipid droplet

Keywords

PTM/Processing

Features

Showing features for signal, chain.

Type
IDPosition(s)Description
Signal1-19
ChainPRO_504213968020-643Polyunsaturated fatty acid lipoxygenase ALOX15

Interaction

Subunit

Interacts with PEBP1; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-95PLAT
Domain95-643Lipoxygenase

Sequence similarities

Belongs to the lipoxygenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    643
  • Mass (Da)
    72,987
  • Last updated
    2024-05-29 v1
  • Checksum
    CA0ECB61F271E33F
MSPRAYYITFFLFARLIAPAPSPSAEVEFQVDVSEYLGRLLFVKLRKRHLLSDDAWFCNWISVQGPGASGNEFRFPCYRWVEGDGILSLPEGTGRTVVDDPQGLFKKHREEELAERRKLYRWGNWKDGLILNIAGATINDLPVDERFLEDKRIDFEASLTKGLADLAIKDSLNILTCWKSLDDFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGTNPMLLRRSVRLPARLEFPPGMGELQAELEKELQQGTLFEADFSLLDGIKANVILCTQQYVAAPLVMLKLQPDGKLLPMAIQLQLPHKGSPPPPLFLPTDPPMTWLLAKCWVRSSDFQLHELHSHLLRGHLVAEVIAVATMRCLPSIHPMFKLLIPHLRYTMEINIRARTGLVSDSGVFDQVVSTGGGGHVELLQRAGAFLTYSSFCPPDDLADRGLLGVKSSFYAQDALRLWEILSRYVEGIVSLHYKTDESVRDDIELQAWCRDITEIGLLGAQDRGFPVTLQSKDQLCHFVTMCIFTCTGQHSSTHLGQLDWYSWVPNAPCTMRLPPPTTKDVTLEKVMATLPNFHQASLQMSITWQLGRRQPIMVALGQHEEEYFSGPEPKAVLKKFREELAALEKDIEIRNAQLDWPYEYLRPSLVENSVAI

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
P27479LOX15_BOVINALOX15663

Keywords

Genome annotation databases

Similar Proteins

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