A0AAA9S438 · A0AAA9S438_BOVIN
- ProteinMethionine synthase
- GeneMTR
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1205 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of a methyl group from methylcob(III)alamin (MeCbl) to homocysteine, yielding enzyme-bound cob(I)alamin and methionine in the cytosol. MeCbl is an active form of cobalamin (vitamin B12) used as a cofactor for methionine biosynthesis. Cob(I)alamin form is regenerated to MeCbl by a transfer of a methyl group from 5-methyltetrahydrofolate. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine.
Catalytic activity
- (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
Cofactor
Protein has several cofactor binding sites:
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 260 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 323 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 324 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 649 | methylcob(III)alamin (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 722-726 | methylcob(III)alamin (UniProtKB | ChEBI) | ||||
Sequence: GDVHD | ||||||
Binding site | 725 | Co (UniProtKB | ChEBI) of methylcob(III)alamin (UniProtKB | ChEBI); axial binding residue | ||||
Sequence: H | ||||||
Binding site | 770 | methylcob(III)alamin (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 774 | methylcob(III)alamin (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 826 | methylcob(III)alamin (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 914 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 1112 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 1167-1168 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: YF |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | cobalamin binding | |
Molecular Function | methionine synthase activity | |
Molecular Function | zinc ion binding | |
Biological Process | methylation | |
Biological Process | pteridine-containing compound metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethionine synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionA0AAA9S438
Proteomes
Organism-specific databases
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 19-338 | Hcy-binding | ||||
Sequence: QDEIEAILQERIMVLDGGMGTMIQQHKLSEEDFRGQEFKDHARPLKGNNDILSITQPSVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEDISLQTGIKRYVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYKEQAKGLLDGGVDILLIETIFDTANAKAALFAVQKLFEEEYVPRPVFISGTIVDKSGRTLSGQTGEAFVISVSHADPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPHVMAMHLKDFAVDGLVNIVGGCCGTTPDHIREIAEAV | ||||||
Domain | 371-649 | Pterin-binding | ||||
Sequence: FVNIGERCNVAGSRRFAKLIMAGNYEEALSVAKMQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIE | ||||||
Domain | 602-699 | B12-binding N-terminal | ||||
Sequence: QTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETK | ||||||
Domain | 712-847 | B12-binding | ||||
Sequence: QGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFE | ||||||
Domain | 863-1205 | AdoMet activation | ||||
Sequence: SLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD |
Domain
Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin.
Sequence similarities
Belongs to the vitamin-B12 dependent methionine synthase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,205
- Mass (Da)133,714
- Last updated2024-05-29 v1
- Checksum14A45DD9750AF361
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A3Q1MEQ8 | A0A3Q1MEQ8_BOVIN | MTR | 1264 | ||
A0AAA9SRP9 | A0AAA9SRP9_BOVIN | MTR | 263 | ||
A0AAA9TNH8 | A0AAA9TNH8_BOVIN | MTR | 1202 | ||
F1N0S3 | F1N0S3_BOVIN | MTR | 1245 | ||
A0AAA9TZN1 | A0AAA9TZN1_BOVIN | MTR | 1119 | ||
A0A3Q1M346 | A0A3Q1M346_BOVIN | MTR | 1214 |
Keywords
- Technical term