A0AA96XWZ3 · A0AA96XWZ3_9PSED

  • Protein
    Isoleucine--tRNA ligase
  • Gene
    ileS
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

1943100200300400500600700800900
TypeIDPosition(s)Description
Binding site567L-isoleucyl-5'-AMP (UniProtKB | ChEBI)
Binding site611ATP (UniProtKB | ChEBI)
Binding site906Zn2+ (UniProtKB | ChEBI)
Binding site909Zn2+ (UniProtKB | ChEBI)
Binding site926Zn2+ (UniProtKB | ChEBI)
Binding site929Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      QOM08_03820

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • P105
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0AA96XWZ3

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

Type
IDPosition(s)Description
Domain27-646Aminoacyl-tRNA synthetase class Ia
Motif58-68'HIGH' region
Motif608-612'KMSKS' region
Domain691-847Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Domain905-932Zinc finger FPG/IleRS-type

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    943
  • Mass (Da)
    105,595
  • Last updated
    2024-03-27 v1
  • Checksum
    0772731B0C2FF901
MTDYKATLNLPDTAFPMKAGLPQREPQILQRWDSIGLYGKLREIGKDRPKFVLHDGPPYANGTIHIGHALNKILKDMIVRSKTLSGFDAPYIPGWDCHGLPIEHKVEVTHGKNLGADKTRELCRAYATEQIEGQKSEFIRLGVLGDFANPYKTMDFKNEAGEIRALAKIVEGGFVFKGLKPVNWCFDCGSALAEAEVEYENKKSSTIDVAFPIADEDKLAAAFGLGKLSKPASIVIWTTTPWTIPANQALNVHPEFNYALVDVGDKLLVLAEELVESCLARYNLEGSVLATAPGSTLELINFRHPFYDRLSPVYLADYVELGAGTGVVHSAPAYGVDDFVTCKKYGMVNDDILNPVQSNGVYVSSLEFFGGQFIWKANPAIVDKLTEVGALLHTTVIEHSYMHCWRHKTPLIYRATAQWFIGMDKQPATGDTLRQRSLKAIEDTKFVPAWGQARLHSMIANRPDWCISRQRNWGVPIPFFLNKESGELHPRTVELMEEVAKRVEVEGIEAWFKMDAAELLGDEAPLYDKISDTLDVWFDSGTTHWHVLRGSHPMGHETGPRADLYLEGSDQHRGWFHSSLLTGCAIDNHAPYRELLTHGFTVDESGRKMSKSLGNVIAPQKVNDTLGADIMRLWVASTDYSGEMAVSEQILQRSADAYRRIRNTARFLLSNLTGFNPATDLLPAEEMLALDRWAVDRTLLLQRELQEHYGEYRFWNVYSKIHNFCVQELGGFYLDIIKDRQYTTGANSKARRSCQTALFHISEALVRWIAPILAFTADELWQYLPGERNESVMLNTWYEGLTELPQGFELDRAYWDRIMAVKAAVNKEMEIQRAAKAVGGNLQAEVTLYAEEALSADLAKLSNELRFVLITSTASVAPLVQAPADAVVTEVSGLKLKIVKSNHTKCARCWHCREDVGVNPEHPEICGRCVDNISGTGEVRHYA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP126693
EMBL· GenBank· DDBJ
WNZ79232.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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