A0AA96XUR3 · A0AA96XUR3_9PSED

  • Protein
    S-adenosylmethionine synthase
  • Gene
    metK
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 divalent ions per subunit.

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site16ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site18Mg2+ (UniProtKB | ChEBI)
Binding site44K+ (UniProtKB | ChEBI)
Binding site57L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site100L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site165-167ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site240ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site240L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site246-247ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site263ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site267ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site271L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionmethionine adenosyltransferase activity
Biological Processone-carbon metabolic process
Biological ProcessS-adenosylmethionine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    S-adenosylmethionine synthase
  • EC number
  • Short names
    AdoMet synthase
  • Alternative names
    • MAT
    • Methionine adenosyltransferase

Gene names

    • Name
      metK
    • ORF names
      QOM08_26190

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • P105
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0AA96XUR3

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer; dimer of dimers.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain6-102S-adenosylmethionine synthetase N-terminal
Region100-110Flexible loop
Domain115-232S-adenosylmethionine synthetase central
Domain234-365S-adenosylmethionine synthetase C-terminal

Sequence similarities

Belongs to the AdoMet synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    396
  • Mass (Da)
    42,506
  • Last updated
    2024-03-27 v1
  • Checksum
    2AF4BDA358DA4751
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKFARVACETLVKTGVAIIAGEVTTSAWVDLEQIVRDVILGIGYNSSDVGFDGATCGVMNIIGKQSPDINQGVDRAKPEDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPWLRPDAKSQVTCRYEGGKVVGIDAVVLSTQHNPEVSYKDLREGVMELIVKHVLPAELLSKDTQFHINPTGQFIIGGPVGDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTGKIGDDKIIKLVREVFDLRPYAITTMLDLLHPMYQDTAAYGHFGRTPQTKTVGDDTFTTFTWEKTDRADALRAAAGL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP126693
EMBL· GenBank· DDBJ
WNZ78123.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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